桂皮醛调控RPS7表达抑制人子宫内膜异位症细胞生长、转移及诱导凋亡  

Cinnamaldehyde inhibits growth,metastasis and induces apoptosis of human endometriotic cells through RPS7

作　　者：詹晓璇 刘城移 彭佳华 刘淑真 李欣 任韵颖 陈丹妮 李佩双[2] 梁瑞宁 Zhan Xiaoxuan;Liu Chengyi;Peng Jiahua;Liu Shuzhen;Li Xin;Ren Yunying;Chen Danni;Li Peishuang;Liang Ruining(Jiangxi University of Traditional Chinese Medicine Graduate School,Nanchang 330004;Dept of Gynecology,Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang 333006;Dept of Gynecology,Second Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang 330012)

机构地区：[1]江西中医药大学研究生院,南昌330004 [2]江西中医药大学附属医院妇科,南昌330006 [3]江西中医药大学第二附属医院妇科,南昌330012

出　　处：《安徽医科大学学报》2025年第3期405-413,共9页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金项目(编号:81160447、81760878);江西省教育厅科学技术研究项目(编号:GJJ2200919);江西省自然科学基金项目(编号:20202BAB206073、20212BAB216066);省级大学生创新创业训练计划项目(编号:S202210412069、S202310412056);江西中医药大学科技创新团队项目(编号:CXTD22013)。

摘　　要：目的研究桂皮醛(CA)对人子宫内膜异位症(EMs)细胞生长、转移及凋亡的影响,并探讨其机制是否与核糖体蛋白S7表达有关。方法将EMs细胞分为对照组、CA组、sh-NC组、CA+sh-RPS7组。CCK-8法和平板克隆形成实验检测细胞增殖;细胞划痕愈合实验和Transwell实验检测细胞迁移与侵袭;Hoechst 33258和流式细胞术检测细胞周期分布与凋亡;并结合Western blot检测增殖细胞核抗原(PCNA)、B淋巴细胞瘤-2(Bcl-2)、Bcl-2关联蛋白(Bax)、E钙黏着蛋白(E-cadherin)、波形蛋白(Vimentin)蛋白表达情况。qRT-PCR和Western blot检测CA干预后RPS7基因和蛋白表达情况。慢病毒转染细胞,以嘌呤霉素药筛建立RPS7过表达的稳定细胞株,Western blot检测转染效率。结合CCK-8法和流式细胞术检测过表达RPS7对细胞增殖与凋亡的影响。结果首先,CA呈量效和时效关系抑制细胞增殖,下调PCNA。CA作用24 h后的IC50值为53.60μmol/L,克隆形成率为25.32%。其次,CA抑制细胞迁移与侵袭,上调E-cadherin和下调Vimentin,细胞迁移率分别为35%和29%,侵袭率为40%。此外,CA通过细胞周期G2/M期阻滞诱导细胞凋亡,细胞凋亡率为25.1%,这与上调Bax和下调Bcl-2有关。最后,CA可显著抑制RPS7表达水平,RPS7表达下调介导了CA抑制细胞增殖和诱导凋亡的效应。结论CA通过下调RPS7的表达抑制细胞生长、转移及诱导凋亡。Objective To investigate the effects of cinnamaldehyde(CA)on the growth,metastasis and apoptosis of human endometriosis(EMs)cells and to explore whether the mechanism is related to ribosomal protein S7(RPS7)expression.Methods Endometriosis cells were divided into control group,CA group,sh-NC group,CA+sh-RPS7 group.Effects of CA on cell growth in human endometriotic cells were determined using Cell Counting Kit-8(CCK-8)and colony formation assay.Effects of CA on cell metastasis were performed by motility assay and Transwell assay.Effects of CA on cell apoptosis were evaluated by Hoechst 33258 staining and flow cytometry.Meanwhile,the levels of PCNA,E-cadherin,Vimentin,Bax and Bcl-2 were evaluated using Western blot in human endometriotic cells with treatment CA.The expression of RPS7 was detected by qRT-PCR and Western blot assay.The RPS7 overexpression of human endometriotic cells was established by cell transfection.CA-mediated effects on cell proliferation and apoptosis were determined by CCK-8 assay and flow cytometry in human endometriotic cells with RPS7 overexpression.Results CA repressed cell growth as well as down-regulated PCNA.The half inhibitory concentration(IC 50)value was 53.60μmol/L after 24 h treatment,and colony formation rate was 25.32%.Additionally,CA inhibited metastasis which was associated with downregulated Vimentin and upregulated E-cadherin.The relative migration rates were 35%and 29%as well as invasion rate was 40%.Further,CA induced apoptosis by cell cycle G 2/M phase arrest and cell apoptosis rate was 25.1%,which related to the up-regulation of of Bax and the down-regulation of Bcl-2.CA inhibited the expression of RPS7 and overexpression of RPS7 promoted cell proliferation and suppressed apoptosis in CA-mediated cells.Conclusion CA inhibits cell growth,metastasis,and induces cell apoptosis by downregulating the expression of RPS7.

关 键 词：桂皮醛 RPS7 子宫内膜异位症 增殖 迁移 侵袭 凋亡 

分 类 号：R965[医药卫生—药理学]