美洲大蠊油脂成分测定及抗氧化活性研究  

Determination of oil composition and antioxidant activity of Periplaneta american

作　　者：查伦贵 顾庆慧 吴明一[2] 胡琳[1] ZHA Lun-gui;GU Qing-hui;WU Ming-yi;HU Lin(Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission&Ministry of Education,School of Ethnic Medicine,Yunnan Minzu University,Kunming 650500,China;State Key Laboratory of Phytochemistry and Natural Medicines,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China)

机构地区：[1]云南民族大学民族药学院民族药资源化学国家民委-教育部重点实验室,云南昆明650500 [2]中国科学院昆明植物研究所植物化学与天然药物全国重点实验室,云南昆明650201

出　　处：《云南民族大学学报(自然科学版)》2025年第2期123-127,共5页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：云南省基础研究计划项目(202301AV070008);云南省院士专家工作站(2020005AF150067).

摘　　要：采用乙醇提取获得美洲大蠊(Periplaneta americana)醇提取物,经分离纯化得到油脂成分,并测定其脂肪酸种类、质量分数及其抗氧化活性.所得油脂经皂化和甲酯化方法处理后,采用GC-MS法分析其脂肪酸种类和质量分数.以维生素E为阳性对照,测定3批油脂清除DPPH自由基的能力.结果显示,3批油脂均鉴定出20种脂肪酸,以油酸、亚油酸、棕榈酸和硬酯酸为主.3批油脂的抗氧化活性均优于维生素E.研究结果为美洲大蠊的综合利用提供了参考依据.The alcoholic extract of Periplaneta americana was obtained by ethanol extraction and its oil composition was purified by separation.Their fatty acids type and content and their antioxidant activity were determined.The oils were treated by saponification and methyl esterification methods,and then analyzed by GC-MS.The ability of three extracted oils to scavenge DPPH free radicals was measured using vitamin E as a positive control.The results show that the three oils contained 20 fatty acids,including oleic,linoleic,palmitic and stearic acids,et al.These oils had stronger antioxidant activity than vitamin E.The research results provide a reference for the comprehensive utilization of American cockroaches.

关 键 词：美洲大蠊 油脂 脂肪酸 抗氧化 

分 类 号：R932[医药卫生—生药学]