阔叶十大功劳治疗肝癌作用机制的生物信息学研究  

Bioinformatics study on the mechanism of Mahonia bealei(Fort.)Carr in the treatment of liver cancer

作　　者：杨霞 杨凯平 张学武 林登梅 张楠楠 李军 YANG Xia;YANG Kai-ping;ZHANG Xue-wu;LIN Deng-mei;ZHANG Nan-nan;LI Jun(School of Basic Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学基础医学院,贵州贵阳550025 [2]贵州中医药大学药学院,贵州贵阳550025

出　　处：《云南民族大学学报(自然科学版)》2025年第2期133-143,共11页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(82060797);贵州中医药大学大学生创新创业训练计划项目(贵中医大创合字(2022)135号).

摘　　要：基于网络药理学和生物信息学探讨了阔叶十大功劳(Mahonia bealei(Fort.)Carr,MB)治疗肝癌的作用机制.首先,利用中药系统药理学数据库与分析平台(TCMSP)数据库获取MB的靶点,并通过基因表达综合数据库(GEO)数据库下载肝癌数据集GSE45267.使用Cytoscape软件和STRING数据库构建蛋白互作网络,并通过David数据库进行基因本体论(GO)和京都基因与基因组(KEGG)富集分析.此外,运用GEPIA、THPA、UALCAN和Kaplan-Meier等数据库进行差异表达、蛋白分析、临床相关性和生存分析,最后通过DS BIOVIA Discovery Studio 2016 v16.1软件进行分子对接.研究筛选出8个hub基因(CCNB1、CCNB2、AURKA、CCNA2、CCNE1、CHEK1、CDK1、TOP2A),这些基因在肝癌组织中显著高表达.生存分析表明,这8个基因与总生存期和无进展生存期显著相关.分子对接结果表明,7个蛋白(CCNB1、AURKA、CCNA2、CCNE1、CHEK1、CDK1、TOP2A)可与MB的成分进行结合.综合网络药理学和生物信息学分析,发现MB通过多个成分、靶点和通路协同作用,可能对肝癌具有治疗潜力.The therapeutic mechanisms of Mahonia bealei(Fort.)Carr(MB)in the treatment of liver cancer are explored based on network pharmacology and bioinformatics.First,the targets of MB were obtained from the Traditional Chinese Medicine Systems Pharmacology(TCMSP)database,and the liver cancer dataset GSE45267 was downloaded from the GEO database.A protein-protein interaction network was constructed using Cytoscape software and the STRING database,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses through the DAVID database.Additionally,differential expression,protein analysis,clinical relevance,and survival analyses were conducted using the GEPIA,THPA,UALCAN,and Kaplan-Meier databases.Molecular docking was performed using DS BIOVIA Discovery Studio 2016 v16.1 software.The study identified eight hub genes(CCNB1,CCNB2,AURKA,CCNA2,CCNE1,CHEK1,CDK1,TOP2A),which were significantly overexpressed in liver cancer tissues.Survival analysis revealed that these eight hub genes were significantly associated with overall survival and progression-free survival.Molecular docking results indicated that seven proteins(CCNB1,AURKA,CCNA2,CCNE1,CHEK1,CDK1,TOP2A)could interact with MB components.Overall,network pharmacology and bioinformatics analyses suggest that MB may exert therapeutic effects on liver cancer through multiple components,targets,and pathways.

关 键 词：网络药理学 生物信息学 阔叶十大功劳 肝癌 作用机制 

分 类 号：R273[医药卫生—中西医结合]