基于“动态比色卡”的胶体金免疫层析试纸检测地表水中己烯雌酚残留  

A colloidal gold immunochromatographic test strip based on“dynamic colorimetric card”for the detection of diethylstilbestrol residues in surface water

作　　者：王庆信 时国庆[2] WANG Qingxin;SHI Guoqing(Longkou Reservoir Management Center,Longkou,265721,China;School of Chemistry and Biological Engineering,University of Science and Technology Beijing,Beijing,100083,China)

机构地区：[1]山东省龙口市水库管理中心,龙口265721 [2]北京科技大学化学与生物工程学院,北京100083

出　　处：《环境化学》2025年第3期1083-1089,共7页Environmental Chemistry

摘　　要：胶体金免疫层析技术可以快速方便的实现目标化合物的可视化检测,因此被认为特别适合现场快速筛查.但胶体金免疫层析试纸检测线的显色受环境温度及湿度影响显著,从而影响了现场可视化检测结果的准确性.为减轻温度、湿度对检测结果的影响,本文以己烯雌酚(DES)单克隆抗体标记胶体金,与NC膜上包被的DES-BSA反应,作为检测线;以鸡免疫球蛋白(IgY)标记胶体金,与NC膜上以3个不同浓度包被的羊抗IgY多克隆抗体反应,作为3条半定量参考线,制备了DES半定量免疫层析检测试纸.该试纸条对DES残留的半定量检测区间为≤5 ng·mL^(−1)、5—10 ng·mL^(−1)、10—20 ng·mL^(−1)、≥20 ng·mL^(−1),在测试的温度、湿度范围内判读结果无显著变化.对地表水样品中DES半定量检测结果与ELISA试剂盒检测结果一致.本研究思路可望用于开发更适合现场检测需求的胶体金免疫层析检测卡.Due to its convenient visual detection of target compounds,colloidal gold immunochromatography technology is particularly suitable for on-site rapid screening;yet,the color development of the colloidal gold immunochromatographic test strip detection line can be significantly impacted by environmental temperature and humidity,thus affecting the accuracy of the on-site visualization detection.To minimize the effects of temperature and humidity on the detection results,a diethylstilbestrol(DES)semi-quantitative immunochromatographic assay strip was prepared by labeling colloidal gold with DES monoclonal antibody and reacting with DES-BSA coated on NC membrane as the detection line;Additionally,three semi-quantitative reference lines were created by labeling colloidal gold with chicken immunoglobulin(IgY)and reacting with sheep anti IgY polyclonal antibodies coated on NC membrane at three different concentrations.This test strip is capable of semi-quantitative analysis of DES concentrations in four ranges:≤5 ng·mL^(−1),5—10 ng·mL^(−1),10—20 ng·mL^(−1),and≥20 ng·mL^(−1),with no significant discrepancies in the results within the tested temperature and humidity ranges.The semi-quantitative detection results of DES in surface water samples are in agreement with the detection results from the ELISA kit.This method is expected to be used to create colloidal gold immunochromatographic detection cards that are more suitable for on-site testing requirements.

关 键 词：胶体金 免疫层析检测 半定量检测 现场检测 己烯雌酚. 

分 类 号：X-1[环境科学与工程] O6[理学—化学]