生活污水中大麻素类物质及其主要生物代谢产物的稳定性研究  

Stability of cannabinoids and the major bio-metabolite in domestic wastewater

作　　者：蔺鑫 陈志香[1] 李先国[1] 张大海[1] LIN Xin;CHEN Zhixiang;LI Xianguo;ZHANG Dahai(School of Chemistry and Chemical Engineering,Ocean University of China,Qingdao,266100,China)

机构地区：[1]中国海洋大学化学化工学院,青岛266100

出　　处：《环境化学》2025年第3期1109-1118,共10页Environmental Chemistry

基　　金：海水养殖生物育种与可持续产出全国重点实验室2023年度开放课题(BRESG202311)支持.

摘　　要：近年来,以污水流行病学为基础的污水验毒技术在大麻滥用评估中应用愈加广泛,大麻中主要活性物质及其生物代谢产物的稳定性是准确评估滥用量的关键.本研究通过标准加入法,研究了避光和光照条件下生活污水中四氢大麻酚(THC)、大麻酚(CBN)、大麻二酚(CBD)的稳定性,并与其主要生物代谢产物四氢大麻酸(THC-COOH)进行了比较;采用多元线性回归以及相关性分析,探讨了样品存储时间(t)、污水水质参数(化学需氧量(COD)、氨氮(NH_(3)-N)、总磷(TP)、总氮(TN)浓度和pH)对THC、CBN、CBD和THC-COOH稳定性的影响.结果表明:避光和光照条件下,18 h内THC、CBN和CBD均降解产生较低浓度的THC-COOH,光照会加快THC、CBN和CBD的降解;24 h内THCCOOH稳定性高于THC、CBN和CBD;4种目标物去除率与t、水质参数的多元线性回归方程中,R^(2)值均大于0.89,在P≤0.05的置信水平上,THC去除率与t具有正相关性,CBN、CBD去除率均与t和NH_(3)-N存在正相关性,THC-COOH去除率与t具有正相关性,与NH_(3)-N存在负相关性.因此,利用生活污水样品进行大麻滥用评估时,以THC-COOH作为目标分析物为佳,取样后避光保存且尽快完成测试(24 h内)对于准确评估大麻滥用量至关重要.In recent years,sewage epidemiology-based drug testing techniques are increasingly used in cannabis abuse assessment.The stability of the main active substances in cannabis and the biometabolite is critical to accurate determination of the concentration and the assessment of cannabis abuse.In this study,the stability of tetrahydrocannabinol(THC),cannabinol(CBN),and cannabidiol(CBD)in domestic wastewater under dark and light irradiation was investigated by the standard addition method.Their stability was compared with that of tetrahydrocannabinoic acid(THCCOOH),which is a major bio-metabolite of cannabinoids in human body.A multiple linear regression and correlation analysis was adopted to evaluate the effects of sample storage time(t),water quality parameters(including chemical oxygen demand(COD),ammonia nitrogen(NH_(3)-N),total phosphorus(TP),total nitrogen(TN)concentration and pH)in domestic wastewater on the stability of THC,CBN,CBD and THC-COOH.The results showed that the degradation of THC,CBN and CBD in domestic wastewater,both in dark and under light irradiation,produced very low concentrations of THC-COOH within 18 h,and the degradation was accelerated by light irradiation.THC,CBN and CBD in domestic wastewater were less stable than THC-COOH within 24 h.The R^(2) values of multiple linear regression model,for correlating the removal rates of the four target compounds with sample storage time(t)and water quality parameters in domestic wastewater,were greater than 0.89 in any case.At a confidence level of P≤0.05,the removal rate of THC had a positive correlation with t,and those of CBN and CBD both had a positive correlation with t and NH_(3)-N,and that of THC-COOH had a positive correlation with t and a negative correlation with NH_(3)-N.Therefore,when using domestic wastewater for cannabis abuse assessment,THC-COOH was better as a target analyte.It is also essential to store samples away from light and complete the test as soon as possible after sampling(preferably within 24 h)for accurate assessment of

关 键 词：四氢大麻酚 大麻酚 大麻二酚 四氢大麻酸 稳定性. 

分 类 号：X-1[环境科学与工程] O6[理学—化学]