Chalcone isomerase gene(OsCHI3)increases rice drought tolerance by scavenging ROS via flavonoid and ABA metabolic pathways  

作　　者：Ting Liu Ling Liu Tianshun Zhou Yinke Chen Huang Zhou Jiahan Lyu Di Zhang Xiwen Shi Dingyang Yuan Nenghui Ye Meijuan Duan 

机构地区：[1]Hunan Provincial Key Laboratory of Rice Stress Biology,College of Agronomy,Hunan Agricultural University,Changsha 410128,Hunan,China [2]Hunan Women’s University,Changsha 410004,Hunan,China [3]State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Hunan Academy of Agricultural Sciences,Changsha 410125,Hunan,China [4]Longping Branch,College of Biology,Hunan University,Changsha 410125,Hunan,China

出　　处：《The Crop Journal》2025年第2期372-384,共13页作物学报(英文版)

基　　金：supported by Science and Technology Innovation Program of Hunan province(2024NK1010,2023NK1010,2023ZJ1080);the National Natural Science Foundation of China(U21A20208).

摘　　要：The chalcone isomerase gene OsCHI,one of the key genes in the flavonoid biosynthesis pathway,plays an important role in rice(Oryza sativa)resistance to abiotic stresses.This study reveals how the chalcone isomerase gene family member OsCHI3 participates in rice responses to drought stress through the regulation of flavonoid biosynthesis.Overexpression of OsCHI3 increased the tolerance of rice to drought stress.In contrast,CRISPR/Cas9-mediated deletion of OsCHI3 reduced the drought tolerance of rice,an effect that is reversed by exogenous ABA treatment.Transcriptomic and physiological biochemical analyses indicated that flavonoids regulated by OsCHI3 not only scavenge reactive oxygen species(ROS)but also increase drought tolerance in rice by stimulating ABA biosynthesis through the regulation of OsNCED1 and OsABA8ox3 expression.These findings demonstrate that OsCHI3 increases drought stress tolerance in rice by activating the antioxidant defense system and the ABA metabolic pathway,providing new clues for drought-resistant rice breeding research.

关 键 词：Chalcone isomerase gene FLAVONOIDS Abscisic acid Drought tolerance RICE 

分 类 号：S511[农业科学—作物学]