The role of imprinted gene ZmFIE1 during maize kernel development  

作　　者：Jing Yang Shengnan Liu Zhen Lin Ning Song Xiaomei Dong Jinsheng Lai Weibin Song Zhijia Yang Jian Chen Qiujie Liu 

机构地区：[1]Frontiers Science Center for Molecular Design Breeding,State Key Laboratory of Maize Bio-breeding,National Maize Improvement Center,Department of Plant Genetics and Breeding,China Agricultural University,Beijing 100193,China [2]Center for Crop Functional Genomics and Molecular Breeding,China Agricultural University,Beijing 100193,China [3]Sanya Institute of China Agricultural University,Sanya 572025,Hainan,China [4]Hainan Yazhou Bay Seed Laboratory,Sanya 572025,Hainan,China [5]College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,Liaoning,China

出　　处：《The Crop Journal》2025年第2期395-405,共11页作物学报(英文版)

基　　金：supported by STI2030-Major Projects(2023ZD04069,Z231100003723004);the National Science Fund for Distinguished Young Scholars(32425041);the Chinese Universities Scientific Fund(2024TC165).

摘　　要：Maize(Zea mays L.)is a globally significant crop essential for food,feed,and bioenergy production.The maize kernel,serving as a primary sink for starch,proteins,lipids,and essential micronutrients,is crucial for enhancing maize yield and quality.Previous studies have established the critical role of Polycomb Repressive Complex 2(PRC2)in regulating kernel development.In this study,we applied a reverse genetics approach to investigate the role of ZmFIE1,the homolog of the PRC2 complex component Extra sex combs(Esc),in maize development.The functional loss of ZmFIE1 significantly reduces embryo size in the early stage but has a relatively small impact on mature kernels.Integrating transcriptional and metabolomic profiling suggests that ZmFIE1 is involved in regulating nutrient balance between the endosperm and embryo.In addition,we demonstrate that ZmFIE1 is maternally expressed,and that the maternal inheritance of the fie1 allele significantly affects the imprinting status of paternally imprinted genes.Overall,our results suggest that ZmFIE1 is a key gene involved in the modulation of embryo development via regulating genomic imprinting and nutrient balance between embryo and endosperm,which provides new insights into the regulation mechanism underlying kernel development.

关 键 词：ZmFIE1 Embryo size Maternally expressed imprinted gene Nutrient metabolism 

分 类 号：S513[农业科学—作物学]