Multi-activity ferruginated carbon quantum dots nanozyme improves wheat seedling growth and Cd tolerance  

作　　者：Zhiwei Lu Yu Li Keyu Chen Songyue Chai Gehong Su Chun Wu Mengmeng Sun Yanying Wang Shiling Feng Ming Hao Hanbing Rao Dengcai Liu 

机构地区：[1]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Sichuan Agricultural University,Chengdu 611130,Sichuan,China [2]College of Science,Sichuan Agricultural University,Ya’an 625014,Sichuan,China [3]Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China [4]College of Chemistry and Life Science,Chengdu Normal University,Chengdu 611130,Sichuan,China [5]College of Life Science,Sichuan Agricultural University,Ya’an 625014,Sichuan,China

出　　处：《The Crop Journal》2025年第2期510-523,共14页作物学报(英文版)

基　　金：supported by the National Natural Science Foundation of China (3237152 and 22104102);Natural Science Foundation of Sichuan Province (2025ZNSFSC0155);Open Project Program (SKL-KF202416) of State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China;Two-Way Support Programs of Sichuan Agricultural University (P202105)

摘　　要：Excessive accumulation of cadmium (Cd) impairs crop growth by inducing oxidative damage through the generation of reactive oxygen species (ROS). In this study, a biocompatible ferruginated carbon quantum dots (Fe-CQDs) nanozyme is developed to target ROS, thereby reducing oxidative damage and improving the absorption and transfer of Cd ions in wheat. Notably, Fe-CQDs exhibit multi-enzyme activities mimicking peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), enabling effective neutralization of active species such as hydroxyl radicals (•OH), hydrogen peroxide (H_(2)O_(2)), and superoxide anions (O_(2)•^(-)). Importantly, root application of 10 mg L^(-1) Fe-CQDs alleviates Cd stress and promotes wheat growth in both hydroponic and soil cultures. Specifically, the levels of O_(2)•^(-), H_(2)O_(2), and malondialdehyde (MDA) in leaf tissues decrease, whereas the non-enzyme antioxidant, reduced glutathione (GSH), increases. Cell wall thickness in the Fe-CQDs-treated group is reduced by 42.4% compared with the Cd group. Moreover, Fe-CQDs enhance the expression of genes related to antioxidants, stress resistance, Cd detoxification, and nutrient transport. Transcriptomic and metabolomic analyses show that Fe-CQDs stimulate the production of flavonoids and regulate the activity of metal transporter genes (YSL, ABC, ZIP) to maintain ROS homeostasis. These findings highlight the potential of Fe-CQDs nanozyme platforms in mitigating oxidative damage and enhancing crop growth, offering new insights into the application of nanobiotechnology in agriculture.

关 键 词：Fe-CQDs nanozyme Oxidative damage Wheat seedling Heavy metal stress NANOBIOTECHNOLOGY 

分 类 号：S512.1[农业科学—作物学]