半夏厚朴汤不同提取部位抗抑郁活性筛选及机制研究  

Screening of antidepressant activity and mechanism of different extracted parts of Banxia Houpo Tang

作　　者：曾元宁 陈雅明 苏慧琳 梁崇豹 王秋红 王倩 ZENG Yuanning;CHEN Ya ming;SU Huilin;LIANG Chongbao;WANG Qiuhong;WANG Qian(Guangdong Engineering Technology Research Center for Standardized Processing of Chinese Materia Medica,School of Chinese Materia Medica,Guangdong Pharmaceutical University,Guangzhou 510006,Guangdong,China;School of Basic Medical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,Guangdong,China;Department of Neurobiology,School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515,Guangdong,China)

机构地区：[1]广东药科大学中药学院广东省中药饮片规范化炮制工程技术研究中心,广东广州510006 [2]广州中医药大学基础医学院,广东广州510006 [3]南方医科大学基础医学院神经生物教研室,广东广州510515

出　　处：《广州医科大学学报》2025年第1期1-8,共8页Academic Journal of Guangzhou Medical University

基　　金：国家青年科学基金(32100819);广东省中医药局科研项目(20221214);广州市基础与应用基础专项(2023A04J1156)。

摘　　要：目的:筛选半夏厚朴汤抗抑郁的活性部位,并初步揭示其抗抑郁的分子机制。方法:半夏厚朴汤提取浓缩后,AB-8大孔树脂柱层析,经梯度乙醇洗脱得到水(W)、30%(30%E)、50%(50%E)和70%乙醇(70%E)部位。强迫游泳实验(FST)、旷场实验(OFT)和脂多糖(LPS)抑郁模型筛选半夏厚朴汤抗抑郁的有效部位。ELISA和实时荧光定量PCR(QPCR)检测小鼠炎性细胞因子表达;蛋白免疫印迹法(Western blotting)测定炎症相关蛋白水平。结果:LPS注射后小鼠表现出抑郁样行为,包括FST中不动时间增加,OFT中运动距离减少;W和70%E部位治疗可逆转LPS引起的抑郁样行为。ELISA和QPCR结果显示:LPS增加小鼠血清、前额叶皮层(PFC)和海马(HPC)中TNF-α、IL-1β、皮质酮(CORT)含量,增加TNF-α、IL-1β mRNA表达;W、70%E部位治疗可以抑制炎性因子的增加。Western blotting显示,W和70%E部位治疗可抑制LPS注射引起的PFC和HPC中AKT、NF-κB p65、TNF-α、JAK2和STAT3蛋白水平增加。结论:半夏厚朴汤发挥抗抑郁作用的活性部位可能是W和70%E组分,可能通过调控AKT/NF-κB/JAK2/STAT3抑制炎症反应发挥抗抑郁作用。Objective:To explore the extracted fractions of Banxia Houpo Decoction for antidepressant activity and elucidate their potential mechanisms.Methods:After extraction and concentration,Banxia Houpo Decoction was transferred to an AB-8 macroporous resin column.The water(W),30%ethanol(30%E),50%ethanol(50%E),and 70%ethanol(70%E)fraction were obtained with gradient ethanol elution.Forced Swim ming Test(FST)and Lipopolysaccharide(LPS)injection induced depression model was used to screened the antidepressant-active fractions.Enzyme-linked immunosorbent assay(ELISA)and quantitative real-time PCR(QPCR)were used to detect the expression of inflammatory cytokines in mice,while Western blotting was employed to deter mine the expression of inflammation-related proteins in PFC and HPC.Results:Mice exhibited depression-like behaviors after LPS injection,characterized by increased immobility time in the FST and reduced locomotor distance in the OFT.Treatment with the W and 70%E fractions reversed these depression-like behaviors induced by LPS.ELISA and QPCR results showed that LPS injection increased TNF-α,IL-1β,corticosterone(CORT)content in serum,prefrontal cortex(PFC),and hippocampus(HPC),and increased the mRNA expression level of TNF-α,IL-1β.Treatment with W and 70%E fraction inhibited the increase in these inflammatory factors.Western blotting analysis revealed that treatment with the W and 70%E fractions suppressed the increased protein levels of AKT,NF-κB p65,TNF-α,JAK2 and STAT3 in the PFC and HPC induced by LPS injection.Conclusion:The active fractions of the antidepressant effect exerted by Banxia Houpo Decoction may be the W and 70%E fractions,which may exert antidepressant effects by inhibiting inflammatory responses through the modulation of the AKT/NF-κB/JAK2/STAT3 signaling pathway.

关 键 词：半夏厚朴汤 活性部位 抑郁症 炎症反应 

分 类 号：R277.7[医药卫生—中医学]