基于重组自交系群体的小麦株高QTL分析  

QTL Mapping for Wheat Plant Height Based on RIL Population

作　　者：周淼平[1] 宋桂成 张鹏[1] 杨学明[1] 张平平[1] 何漪 ZHOU Miaoping;SONG Guicheng;ZHANG Peng;YANG Xueming;ZHANG Pingping;HE Yi(Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014,China)

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏南京210014

出　　处：《麦类作物学报》2025年第4期421-431,共11页Journal of Triticeae Crops

基　　金：国家小麦产业体系项目(CARS-03-57);江苏省重点研发计划项目(BE2021375)。

摘　　要：为了挖掘更多与小麦株高相关且适合标记辅助育种需求的QTL,采用长江中下游麦区骨干亲本扬麦158和西风配制重组自交系群体,使用55K芯片技术分析群体基因型并构建遗传连锁图,联合4个年度群体株高表型资料,对影响群体株高的QTL进行分子定位。结果表明,群体遗传连锁图含有3830个SNP标记,覆盖2784.9 cM,标记间平均距离0.7 cM;21条染色体共定位了39个可重复的与株高相关QTL,其中18个QTL为2个年度可重复定位,8个QTL为3个年度可重复定位,13个QTL为4个年度可重复定位,单个QTL可解释3.2%~13.6%的株高表型变异。4个年度可重复定位的QTL分别来自西风的1B、1D、4A、4B、4D、5A、5B、7A染色体以及扬麦158的3A、3D、6A、7B、7D染色体,其中扬麦158贡献的QPh·jaas-3A.3和QPh·jaas-7B.1分别解释8.4%~13.4%以及9.1%~13.1%的株高变异。这些株高QTL位点有望为长江中下游麦区小麦分子标记辅助育种提供帮助。Wheat plant height is an important agronomic trait affecting wheat yield,harvest index and lodging resistance of the plants,and cultivating wheat with proper plant height for production needs is one of the important goals of breeders.In order to explore more QTLs related to wheat plant height and suitable for marker-assisted breeding,the recombinant inbred line population was developed by Yangmai 158 and Xifeng,the backbone parents of the wheat area in the middle and lower reaches of the Yangtze River.The population genotype was analyzed by 55K SNP array and the genetic linkage map was constructed by JoinMap software.Combining the phenotypic data of plant height in four years,the QTLs associated with wheat plant height were mapped in the chromosomes.The result showed that the genetic linkage map containing 3830 SNPs distributed in 2784.9 cM of the wheat genome and the average distance between markers was 0.7 cM.A total of 39 repeatable plant height-related QTLs were detected on 21 chromosomes of wheat,of which 18 QTLs were only revealed in 2 years;8 QTLs were found in 3-year data analysis,and 13 QTLs were mapped in 4 years.A single QTL explained 3.2%-13.6%of the plant height phenotypic variation.The stable QTLs mapped in the four years were individually derived from chromosomes 1B,1D,4A,4B,4D,5A,5B,and 7A of Xifeng and chromosomes 3A,3D,6A,7B,and 7D of Yangmai 158.The QPh·jaas-3A.3 and QPh·jaas-7B.1 contributed by Yangmai 158 could explain 8.4%-13.4%and 9.1%-13.1%of the plant height phenotypic variation,which are helpful for molecular marker-assisted breeding of wheat in the middle and lower reaches of the Yangtze River.

关 键 词：小麦 株高 重组自交系 QTL 

分 类 号：S512.1[农业科学—作物学] S330