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作 者:马本兴 崔赫 赵海波 王营 郑豫川 高翔[1] 杨明明[1] 赵万春[1] 李晓燕[1] 董剑[1] MA Benxing;CUI He;ZHAO Haibo;WANG Ying;ZHENG Yuchuan;GAO Xiang;YANG Mingming;ZHAO Wanchun;LI Xiaoyan;DONG Jian(College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China)
机构地区:[1]西北农林科技大学农学院,陕西杨凌712100
出 处:《麦类作物学报》2025年第4期440-447,共8页Journal of Triticeae Crops
基 金:陕西省重点研发计划项目(22NY-184)。
摘 要:为给小麦遗传改良和新品种选育提供丰富材料,对161份硬粒小麦与普通小麦杂交后代F6株系的农艺性状、条锈病抗性和品质性状进行检测,并对主要性状进行相关分析和主成分分析,利用0.1K基因芯片对其进行功能基因检测。结果发现,161份株系的农艺和品质性状变异较大,遗传多样性较丰富,大多数被测性状出现超亲现象。对被测性状进行主成分分析,综合排名前三株系的编号分别为137、98和155。对被测性状进行Q型聚类分析,161份株系可划分为五个类群,其中第Ⅲ类群综合表现最好,包括60个株系,具有高抗条锈病、小穗排列紧密、蛋白质含量高且质量好等特性,10号株系表现最好。利用0.1K芯片分析群体功能基因的分布,结合性状表现分析发现,F6群体后代中含有TaCwi-A1、Qpht-2D、Yr17基因,分别对提高千粒重、降低株高、抗条锈病有正向效应。主成分分析排名前列和Q聚类分析第三类群性状较好的株系可用于陕西小麦农艺与品质性状的遗传改良。In order to provide abundant materials for genetic improvement and breeding of new wheat varieties,the agronomic traits,stripe rust resistance and quality traits of 161 F 6 lines derived from a crossing between durum wheat and common wheat were determined;the correlation analysis and principal component analysis of the main traits were carried out,and 0.1K gene array was used for the functional gene analysis.The results showed that the top three lines were the number of 137,98 and 155,respectively.The Q-type cluster analysis of the tested traits showed that the 161 lines were divided into five groups,among which groupⅢshowed the best comprehensive performance,including 60 lines,with high resistance to stripe rust,tight spikelet arrangement,high protein content and good quality,and the number 10 line was the most representative.The distribution of population functional genes was analyzed by 0.1K array,and it was found that the offspring of F 6 population contained TaCwi-A1,Qpht-2D and Yr17 genes,which had positive effects on increasing 1000-grain weight,reducing plant height,and resisting to stripe rust.The agronomic and quality traits of the 161 lines varied greatly,and the genetic diversity was rich.The top three lines that the number of 137,98 and 155,and the third group from Q-type can be used for genetic improvement of wheat agronomic and quality traits in Shaanxi.
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