麦田多花黑麦草种群对ACCase及ALS类抑制剂的抗性及土壤处理药剂筛选  

Resistance Levels of Lolium multiflorum Populations to ACCase and ALS Inhibitors and the Screening of Pre-Emergence Herbicides in Wheat Fields

作　　者：王慧 戴魏真 许锦程 贾增坡[3] 毕亚玲 WANG Hui;DAI Weizhen;XU Jincheng;JIA Zengpo;BI Yaling(College of Resources and Environment,Anhui Science and Technology University,Fengyang,Anhui 233100,China;College of Agricultural,Anhui Science and Technology University,Fengyang,Anhui 233100,China;Anhui Fengle Agrochemical Co.Ltd.,Hefei,Anhui 231600,China)

机构地区：[1]安徽科技学院资源与环境学院,安徽凤阳233100 [2]安徽科技学院农学院,安徽凤阳233100 [3]安徽丰乐农化有限责任公司,安徽合肥231600

出　　处：《麦类作物学报》2025年第4期547-561,共15页Journal of Triticeae Crops

基　　金：国家重点研发计划项目(2023YFD1400501);安徽省科技重大专项(202203a06020016)。

摘　　要：为明确多花黑麦草(Lolium multiflorum)对乙酰辅酶A羧化酶(ACCase)及乙酰乳酸合成酶(ALS)类除草剂的抗性现状,在河南省驻马店市麦田多花黑麦草发生严重区域采集了50个种群,采用整株生物测定法分别测定了其对唑啉草酯、炔草酯、啶磺草胺的抗性水平;另选择异丙隆、吡氟酰草胺、氟噻草胺、砜吡草唑、吡酰·异丙隆、啶磺草胺对其进行生物活性测定;并将砜吡草唑、氟噻草胺、啶磺草胺与吡酰·异丙隆混配开展田间药效评价。结果表明,供试多花黑麦草种群中,8个种群对唑啉草酯表现高水平抗性,GR50值(抑制杂草地上部分鲜重50%时所使用的除草剂剂量)在146.67~456.15 g(a.i.)·hm^(-2)之间,抗性指数(RI)在10.68~33.20之间,占供试种群的16%;31个种群对炔草酯表现高水平抗性,GR50在101.82~446.40 g(a.i.)·hm^(-2)之间,RI在10.36~45.41之间;16个种群对啶磺草胺表现高水平抗性,占总数的32%。除吡氟酰草胺和啶磺草胺外,其余4种除草剂(异丙隆、氟噻草胺、砜吡草唑、吡酰·异丙隆)对SC-3(最高水平抗性种群)的生物活性均较高,GR90分别为1089.36、144.43、51.42、568.69 g(a.i.)·hm^(-2)。从田间药效看,砜吡草唑与吡酰·异丙隆混配在供试剂量下对多花黑麦草的防效较好,鲜重防效可达94.27%~100%,且对小麦安全。因此,对于多花黑麦草发生严重的麦田,可采用该配方进行防治。In order to clarify the current resistance status of Lolium multiflorum to the herbicides of acetyl CoA carboxylase(ACCase)and acetolactate synthase(ALS),a total of 50 populations were collected in the severe area of L.multiflorum in Zhumadian City,Henan Province and the resistance levels to pinoxaden,clodinafop-propargyl and pyroxsulam were determined by the whole-plant bioassay method.In addition,isoproturon,diflufenican,flufenacet,pyroxasulfone,diflufenican+isoproturon,and pyroxsulam were selected for bioactivity determination,and field efficacy evaluation were conducted with pyroxasulfone,flufenacet,and pyroxsulam mixed with diflufenican+isoproturon.The results showed that eight populations of L.multiflorum had high resistance to pinoxaden.The GR 50(herbicide dose causing growth reduction of fresh weight by 50%)values ranged from 146.67 g(a.i.)·hm^(-2)456.15 g(a.i.)·hm^(-2),and the resistance index(RI)ranged from 10.68 to 33.20,accounting for 16%of the tested populations.Thirty-one populations showed high level resistance to clodinafop-propargyl,with GR 50 ranging from 101.82 g(a.i.)·hm^(-2)446.40 g(a.i.)·hm^(-2),and RI ranging from 10.36 to 45.41;sixteen populations had high level resistance to pyroxsulam,accounting for 32%of the total.In the bioactivity assays,except for diflufenican and pyroxsulam,the other herbicides(isoproturon,flufenacet,pyroxasulfone,diflufenican+isoproturon)showed high activity against the SC-3 population(the highest level of resistance population),with GR 90 values of 1089.36,144.43,51.42 and 568.69 g(a.i.)·hm^(-2),respectively.Field efficacy results indicated that the mixture of pyroxasulfone and diflufenican+isoproturon provided good control of L.multiflorum at the supplied dose,with fresh weight control efficacy ranging from 94.27%to 100%,and safe to wheat.Therefore,the mixture of pyroxasulfone and diflufenican+isoproturon can be used to control the serious occurrence of L.multiflorum in wheat fields.

关 键 词：多花黑麦草 抗药性 生物活性 药剂筛选 防除效果 

分 类 号：S512.1[农业科学—作物学] S481.4