胡杨PeMAX2对拟南芥镉吸收和耐受能力的影响  

Effects of Populus euphratica PeMAX2 on cadmium uptake and tolerance in Arabidopsis

作　　者：安珂悦 马思圆 李静[1] 赵瑞[1] 陈少良[1] 赵楠 周晓阳 尧俊 AN Keyue;MA Siyuan;LI Jing;ZHAO Rui;CHEN Shaoliang;ZHAO Nan;ZHOU Xiaoyang;YAO Jun(State Key Laboratory of Efficient Production of Forest Resources,College of Biological Science and Biotechnology,Beijing Forestry University,Beijing 100083,China;Public Analysis and Testing Center,Beijing Forestry University,Beijing 100083,China;Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangdong Academy of Forestry,Guangzhou 510520,China)

机构地区：[1]北京林业大学生物科学与技术学院,林木资源高效生产全国重点实验室,北京100083 [2]北京林业大学实验室与设备管理处,北京100083 [3]广东省森林培育与保护利用重点实验室,广东省林业科学研究院,广东广州510520

出　　处：《河南农业大学学报》2025年第2期233-242,共10页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(32371828,32071730,317706643);高等学校学科创新引智计划项目(111 Project,B130007);北京市自然科学基金项目(6182030,6172024);中央高校基本科研业务专项(2019ZY25)。

摘　　要：【目的】研究胡杨PeMAX2转录因子调控植物响应镉胁迫的生理和分子机制,对挖掘、筛选和鉴定重金属抗性相关基因具有重要意义。【方法】以过表达PeMAX2拟南芥(PeMAX2-OE1和PeMAX2-OE2)、野生型(WT)和Atmax2突变体拟南芥为试验材料,利用培养基培养的幼苗(7 d)及土培大苗(30 d)研究了PeMAX2对植物耐镉性的调控作用。【结果】镉处理后,在培养基的拟南芥幼苗中,PeMAX2过表达株系的相对电导率明显低于WT和Atmax2,与其抗氧化能力增强有关。在镉胁迫下,转基因株系超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均有明显升高且幅度显著高于WT和Atmax2,抗氧化酶转录水平的变化趋势与酶活性相似。土培拟南芥镉处理后转基因株系的叶绿素含量下降幅度低于WT和Atmax2。另外,转基因株系的光合和荧光参数也均高于WT和Atmax2。【结论】过表达胡杨PeMAX2提高了拟南芥对镉的吸收能力,转基因拟南芥通过提高抗氧化酶活性增强对镉的耐受性。【Objective】This study investigated the physiological and molecular mechanisms of MAX2(MORE AXILLARY BRANCHES2)in Populus euphratica in the regulation of plant response to cadmium stress,which is of great significance for the mining,screening and identification of key genes related to heavy metal tolerance.【Method】Arabidopsis thaliana overexpressing PeMAX2(PeMAX2-OE1 and PeMAX2-OE2),wild-type(WT)and Atmax2 mutant were used as experimental materials,the role of PeMAX2 in regulating cadmium tolerance in plants was determined by using seven-day-old seedlings grown on 1/2 MS medium and 30-day-old soil-cultured seedlings.【Result】The relative electrical conductivity of the PeMAX2-overexpressed lines were significantly lower than those of the WT and the Atmax2 mutants in Arabidopsis thaliana seedlings after cadmium treatment,which is due to the improved antioxidant capacity.The superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)activities of the transgenic lines increased remarkably,which was significantly higher than that of WT and Atmax2.Under cadmium stress,the cadmium-altered transcription of the antioxidant enzymes resembles the pattern of enzyme activity.In addition,the chlorophyll content of the transgenic line decreased less than that of WT and Atmax2 after being treated CdCl2 for 10 days of soil-cultured Arabidopsis thaliana seedlings.The photosynthetic and chlorophyll fluorescence parameters of transgenic lines were higher than those of WT and mutants.【Conclusion】The overexpression of P.euphratica PeMAX2 gene increased cadmium uptake in Arabidopsis thaliana,and transgenic plants improved Cd tolerance by increasing antioxidant enzyme activity.

关 键 词：胡杨 PeMAX2 拟南芥 重金属 镉胁迫 光合作用 抗氧化酶 

分 类 号：Q945.78[生物学—植物学]