基于TMAO/NF-κB轴探讨芪苈强心胶囊改善心肌梗死后心功能的机制研究  

Study on the mechanism of Qili Qiangxin Capsule improving cardiac function after myocardial infarction based on TMAO/NF-κB axis

作　　者：焦雪妍 刘新灿[1] 陈云[1] 兰真真 张艺凡 刘璐瑶 JIAO Xueyan;LIU Xincan;CHEN Yun;LAN Zhenzhen;ZHANG Yifan;LIU Luyao(Heart Center,the First Affiliated Hospital of Henan University of Chinese Medicine,Henan Zhengzhou 450003,China;The First Clinical Medical College of Henan University of Chinese Medicine,Henan Zhengzhou 450003,China)

机构地区：[1]河南中医药大学第一附属医院心脏中心,河南郑州450003 [2]河南中医药大学第一临床医学院,河南郑州450003

出　　处：《中国医院药学杂志》2025年第6期607-613,共7页Chinese Journal of Hospital Pharmacy

基　　金：国家中医药传承创新专项(编号:2023ZXZX1156);河南省中医药科学研究重点专项(编号:2023ZY1001);河南省青年人才托举工程项目(编号:2021HYTP057)。

摘　　要：目的:基于TMAO/NF-κB轴探讨芪苈强心胶囊改善心肌梗死后心功能的作用机制。方法:通过结扎左冠状动脉前降支构建大鼠急性心肌梗死模型,利用超声心动图评估大鼠左室射血分数(left ventricular ejection fraction,LVEF)、左室短轴缩短率(left ventricular fractional shortening,LVFS)、左室舒张末期内径(left ventricular internal diameter in diastole,LVIDD)、左室收缩末期内径(left ventricular internal diameter in systolic,LVIDS)。观察大鼠心脏组织形态学改变和纤维化程度,检测血清氧化三甲胺(trimethylamine oxide,TMAO)含量、可溶性生长刺激表达基因2含量(soluble growth stimulation expressed gene2,sST2)浓度,检测NF-κb、p-NF-κb、TNFα、IL-6、IL-1β蛋白表达情况。结果:与急性心肌梗死组相比,芪苈强心胶囊组大鼠血清TMAO、sST2浓度、心肌组织p-NF-κB/NF-κB及TNFα、IL-6和IL-1β蛋白表达降低,LVEF、LVFS、LVIDD、LVIDS、心脏病理形态及纤维化水平均得到显著改善(P<0.05);与高胆碱饮食组大鼠相比,芪苈强心胶囊+高胆碱饮食组大鼠血清TMAO、sST2浓度、心肌组织p-NF-κB/NF-κB及TNFα、IL-6和IL-1β蛋白表达降低,LVEF、LVFS、LVIDD、LVIDS、心脏病理形态及纤维化水平均得到显著改善(P<0.05)。结论:芪苈强心胶囊可能通过抑制TMAO/NF-κB轴,减少炎症因子TNF-α、IL-6、IL-1β的释放,从而改善心肌梗死后的心功能。OBJECTIVE To explore the mechanism of Qili Qiangxin(QLQX)Capsule in improving cardiac function after myocardial infarction based on trimethylamine N-oxide(TMAO)/nuclear factor kappa-B(NF-κB)axis.METHODS A rat model of acute myocardial infarction(AMI)was created by ligating the left coronary artery's anterior descending branch.Echocardiography was used to assess left ventricular ejection fraction(LVEF),left ventricular fractional shortening(LVFS),left ventricular internal dimension in diastole(LVIDD),and systole(LVIDS).Morphological changes and fibrosis levels in cardiac tissue were examined,and serum levels of trimethylamine oxide(TMAO)and soluble ST2(sST2)were measured.Meanwhile,protein expression levels of NF-κb,phosphorylated NF-κb(p-NF-κb),tumor necrosis factorα(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β)were detected.RESULTS Compared to the AMI group,the QLQX group showed significant reductions in serum TMAO and sST2 levels,and protein expressions of p-NF-κB/NF-κB,TNF-α,IL-6,and IL-1βin myocardial tissues,along with notable improvements in LVEF,LVFS,LVIDD,LVIDS,cardiac morphology and fibrosis(P<0.05).Similarly,rats in the QLQX group demonstrated significantly lower TMAO,sST2,p-NF-κB/NF-κB,TNF-α,IL-6,and IL-1βlevels and improved echocardiographic and histopathologic parameters compared to the Choline group(P<0.05).CONCLUSION QLQX Capsule may improve cardiac function after myocardial infarction by reducing inflammatory markers TNF-α,IL-6,and IL-1βthrough inhibition of the TMAO/NF-κB axis.

关 键 词：心肌梗死 芪苈强心胶囊 TMAO/NF-κB 

分 类 号：R282[医药卫生—中药学] R966[医药卫生—中医学]