基于DESI-MSI分析大麦芽碱在大鼠体内组织的空间分布特征  

Spatial distribution characteristics of hordeine in rat tissues analyzed by DESI-MSI

作　　者：周雨婕 张莉 汪薇 王彬 徐慧兰 陈永刚 ZHOU Yujie;ZHANG Li;WANG Wei;WANG Bin;XU Huilan;CHEN Yonggang(College of Pharmacy,Hubei University of Chinese Medicine,Hubei Wuhan 430070,China;Wuhan Center for Clinical Laboratory,Hubei Wuhan 430064,China;Inspection and Testing Institute 1,Hubei Provincial Institute for Food Supervision and Test,Hubei Wuhan 430073,China)

机构地区：[1]湖北中医药大学药学院,湖北武汉430070 [2]武汉市临床检验中心,湖北武汉430064 [3]湖北省食品质量安全监督检验研究院检验1所,湖北武汉430073

出　　处：《中国医院药学杂志》2025年第6期628-633,共6页Chinese Journal of Hospital Pharmacy

基　　金：湖北省重点研发计划项目(编号:2023BCB033);武汉市重点项目(编号:S202103030078);武汉市中医药科研项目(编号:WZ22B03)。

摘　　要：目的:利用解析电喷雾电离质谱成像(desorption electrospray ionization mass spectrometry imaging,DESI-MSI)技术,可视化分析大麦芽碱化合物口服给药后在大鼠体内的组织分布,并具体分析大麦芽碱随时间延长在肾脏组织的表征分布特征。方法:以大麦芽碱为目标小分子化合物,以SD雌性大鼠为实验动物,以50 mg·kg^(–1)大麦芽碱水溶液口服给药,一定时间后取出大鼠心脏、肾脏、大脑、垂体和脾脏等器官。利用DESI-MSI技术分析同时段给药的目标化合物在体内的组织分布;进一步分析给药后0.5、1、1.5、4.6、6.6、12 h的肾脏中大麦芽碱化合物在组织的分布特征变化。结果:质谱成像结果可视化展现出大麦芽碱在各组织结构中分布的不同丰度,大脑中大麦芽碱的分布强度较弱,但可证明药物能透过血脑屏障到达作用靶点垂体;肾脏是各组织中信号强度最强,大麦芽碱分布变化最明显的组织,可通过肾脏各时间点的质谱成像图推断大麦芽碱的作用途径。结论:利用DESI-MSI技术在组织水平上探索大麦芽碱化合物的分布特征,实现了大鼠体内药物原位可视化分析,详细探讨了大麦芽碱作为药物候选化合物的潜在应用前景,为其在药物开发和治疗方面的应用提供了深入的了解和展望。OBJECTIVE To visualize and analyze the spatial tissue distribution of hordeine compound after oral administration in rats using desorption electrospray ionization mass spectrometry imaging(DESI-MSI)technique,and to specifically investigate the characteristic distribution of hordeine in kidneys tissues over time.METHODS Hordeine was selected as the target small molecule compound.Female Sprague-Dawley(SD)rats were orally administered with 50 mg·kg^(–1)hordeine aqueous solution.After a certain time,rat organs,including the heart,kidney,brain,pituitary,and spleen were harvested,frozen,and sectioned.DESI-MSI technique was employed to analyze the tissue distribution of the target compound at various time points postadministration.Furthermore,the distribution characteristics of hordeine in kidneys tissue sections at 0.5 h,1 h,1.5 h,4.6 h,6.6 h,and 12 h post-administration were analyzed.RESULTS The mass spectrometry imaging visualization showed varying abundances of hordeine in different tissue structures.Although its distribution in the brain was relatively weak,hordeine was able to cross the blood-brain barrier and reached its target,the pituitary gland.The kidneys exhibited the strongest signal and the most significant distribution changes among all tissues.The mechanism of action of hordeine could be inferred from the mass spectrometry imaging of the kidneys at various time points.CONCLUSION DESI-MSI technique facilitates the exploration of the distribution characteristics of hordeine compound at the tissue level.This study achieved in situ visualization analysis of drugs in rats,providing detailed insights into the potential application prospects of hordeine as a drug candidate compound.It offers a deeper understanding and outlook for its application in drug development and therapy.

关 键 词：大麦芽碱 解析电喷雾电离质谱成像技术 空间组学 药物原位可视化分析 组织分布特征 

分 类 号：R285[医药卫生—中药学]