仿生磷脂聚合物及纳米银双功能涂层的构建及防污抗菌性能  

Construction of dual-functional coating of biomimetic phospholipid polymer and silver nanoparticles and its antifouling and antibacterial properties

作　　者：魏佳峰 刘星星 贾康乐 苏秋萍 李欢玲 余龙飞 WEI Jiafeng;LIU Xingxing;JIA Kangle;SU Qiuping;LI Huanling;YU Longfei(School of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510006,Guangdong,China;Guangdong Academy of Sciences,Institute of Chemical Engineering,Guangzhou 510665,Guangdong,China)

机构地区：[1]广东工业大学轻工化工学院,广东广州510006 [2]广东省科学院化工研究所,广东广州510665

出　　处：《精细化工》2025年第4期828-836,928,共10页Fine Chemicals

基　　金：揭阳市(扬帆计划)引进创新创业团队和领军人才项目(2022yfgh007);2023年汕头市精细化工企业引进科技领军人才团队及进口替代技术攻关专项资金项目(230905135015574)。

摘　　要：以多巴胺甲基丙烯酰胺(DMA)和2-甲基丙烯酰氧乙基磷酸胆碱(MPC)为单体,通过自由基聚合制备了两性离子共聚物(PMPCD),再以多巴胺盐酸盐(DA)为锚固剂将PMPCD接枝到基材表面,形成了防污涂层。最后,聚多巴胺(PDA)将银离子原位还原成纳米银(AgNPs),实现了PDA/PMPCD-AgNPs涂层的防污-杀菌双功能。采用FTIR、1HNMR、SEM、XPS对PDA/PMPCD-AgNPs涂层进行了表征,测试了其黏附力和水接触角。通过抗蛋白质、抗血小板吸附、溶血和细胞毒性实验考察了PDA/PMPCD-AgNPs涂层的生物相容性。结果表明,PDA/PMPCD-AgNPs涂层具有优异的亲水性(平均水接触角25.0°~34.0°)、抗菌性和生物相容性;PDA/PMPCD-AgNPs涂层对大肠杆菌和金黄色葡萄球菌的抗菌率分别高达98%及95%以上;抗蛋白质吸附率>90%,同时能减少血小板的吸附和活化,并展现出较低的溶血率及优异的细胞活性。Zwitteronic copolymer(PMPCD)was synthesized from free radical copolymerization of dopamine methacrylamide(DMA)and 2-methacryloyloxyethyl phosphorylcholine(MPC),and grafted onto substrate surface with dopamine(DA)as anchor agent to form an antifouling coating.Dual-functional PDA/PMPCD-AgNPs coating with antifouling and antibacterial properties was then obtained from in situ reduction of silver ions to silver nanoparticles(AgNPs)by polydopamine(PDA),and characterized by FTIR,1HNMR,SEM and XPS,with its adhesion and water contact angle analyzed.The biocompatibility of PDA/PMPCD-AgNPs coating was further evaluated by anti-protein,anti-platelet adsorption,hemolysis and cytotoxicity tests.The results showed that the PDA/PMPCD-AgNPs coating exhibited excellent hydrophilicity(average water contact angle 25.0°~34.0°),antibacterial property and biocompatibility.The antibacterial rates of PDA/PMPCD-AgNPs coating on Escherichia coli and Staphylococcus aureus were 98%and 95%,respectively.Meanwhile,the coating displayed an anti-protein adsorption rate>90%,reduced platelet adsorption and activation,and low hemolysis rate as well as excellent cell activity.

关 键 词：两性离子聚合物 纳米银 表面改性 防污性能 抗菌性能 生物工程 

分 类 号：R318.08[医药卫生—生物医学工程]