Diversification of flavonoid accumulation among ecotypes of Agriophyllum squarrosum(L.)Moq.in response to drought stress  

作　　者：ZHAO Pengshu YAN Xia QIAN Chaoju MA Guorong FANG Tingzhou YIN Xiaoyue ZHOU Shanshan LIAO Yuqiu SHI Liang FAN Xingke Awuku IBRAHIM MA Xiaofei 

机构地区：[1]Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands,Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou 730000,China [2]Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province,Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou 730000,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Key Laboratory of Inland River Ecohydrology,Cold and Arid Regions Environmental and Engineering Research,Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou 730000,China [5]Gulang County Sand Prevention and Control Technology Promotion Center,Wuwei 733100,China

出　　处：《Journal of Arid Land》2025年第4期538-559,共22页干旱区科学(英文版)

基　　金：supported by the National Natural Science Foundation of China(32271695);the Science and Technology Program of Gulang County,Gansu Province(GL2302YFN006);the Youth Science and Technology Talent Innovation Project of Lanzhou City(2023-QN-140);the Strategic Biological Resources Program of Chinese Academy of Sciences(KFJ-BRP-007-015);the Development of Scientific and Technological Innovation Special Fund Competitive Project of Gansu Province(Y939BD1001).

摘　　要：Agriophyllum squarrosum(L.)Moq.,commonly known as sandrice,is an annual medicinal plant prevalent in the dunes across China's deserts.A garden trial revealed that flavonoid content varies among sandrice ecotypes due to long-term local adaptation to water variability.To investigate how sandrice responds to drought stress through the molecular metabolic regulation of flavonoids,we employed transcriptomic and metabolomic analyses during a 9-d ambient drought stress,examining three ecotypes along a precipitation gradient.The three ecotypes located in Dengkou(DK)County,Dulan(DL)County,and Aerxiang(AEX)village of northern China,which had 137,263,and 485 mm precipitation,respectively.Soil moisture content was 4.04%after drought stress,causing seedlings of the three sandrice ecotypes to display collapsed structures,yellowing leaves,wilting,and curling.Among these,DL exhibited superior drought tolerance,in which plant height increase(PHI)and leaf area(LA)were significantly higher than those of DK and AEX.Flavonoid-targeted metabolomics identified that rutin,isoquercitrin,and astragalin constituted over 95.00%of the 15 flavonoid metabolites detected.A total of 12 differentially accumulated flavonoids(DAFs)were found,with rutin being the most abundant(1231.57-2859.34 ng/100 mg fresh weight(FW)),showing a gradual increase along the precipitation gradient.Transcriptomic analysis revealed 14 common differentially expressed genes(DEGs)associated with flavonoid synthesis among the three ecotypes.Integrative analysis of DEGs and DAFs indicated that sandrice adapts to drought stress by activating different flavonoid synthesis pathways.In DK,the dihydrokaempferol-dihydroquercetin pathway,regulated by flavonoid 3'-monooxygenase(CYP75B1),likely enhances drought adaptation.In AEX,transcriptional repression by O-methylatransferase(OMT)shifts the metabolic flux from the quercetin-isorhamnetin pathway to the quercetin-isoquercetin-rutin pathway in response to drought.DL,the most droughttolerant ecotype,appears to activate the naringenin-

关 键 词：drought tolerance ECOTYPE flavonoid medicinal plant RUTIN 

分 类 号：Q945.78[生物学—植物学] S511[农业科学—作物学]