壮医药线点灸抑制核因子-κB通路及炎症因子改善带状疱疹后遗神经痛大鼠神经炎症的机制研究  

Zhuang medicine thread moxibustion inhibits NF-κB pathway and inflammatory factors mechanism study on the improvement of neuroinflammation in PHN rats

作　　者：冼思彤 林采玥 施学丽 韦丽丽 周玲瑶 范小婷 黄光田 李金溢[3] 林辰 XIAN Sitong;LIN Caiyue;SHI Xueli;WEI Lili;ZHOU Lingyao;FAN Xiaoting;HUANG Guangtian;LI Jinyi;LIN Chen(College of Zhuang Medicine,Guangxi University of Traditional Chinese Medicine,Nanning 530000,China)

机构地区：[1]广西中医药大学壮医药学院,南宁530000 [2]广西中医药大学瑞康临床医学院,南宁530000 [3]广西中医药大学第一附属医院仁爱分院治未病科

出　　处：《环球中医药》2025年第3期397-404,共8页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(82160947、82360982);国家非物质文化遗产项目(Ⅸ-18);广西研究生教育创新计划(YCBZ2023151);广西高校中青年教师科研基础能力提升项目(2024KY0296);广西自然科学基金(2023GXNSFBA026050)。

摘　　要：目的探讨壮医药线点灸对树脂毒素诱导的带状疱疹后遗神经痛(postherpetic neuralgia,PHN)大鼠的治疗作用及机制。方法75只SD雄性大鼠,随机分为盐水组、模型组、点灸组、吡咯烷二硫代甲酸铵(pyrrolidinedithiocarbamate,PDTC)组、PDTC+点灸组,每组15只。除盐水组外,其余大鼠均腹腔注射树脂毒素建立PHN样大鼠模型。点灸组于造模成功后隔日予壮医药线点灸治疗,隔日点灸一次,10次为一疗程;PDTC组建模成功后予以腹腔注射核因子-κB(nuclear factor kappa-B,NF-κB)抑制剂PDTC 50 mg/kg,每日1次;PDTC+点灸组在建模成功后腹腔注射PDTC,并采用壮医药线点灸疗法治疗。定期测量各组大鼠行为学改变情况,同时对背根神经节组织进行实时荧光定量PCR、酶联免疫吸附法、RNA测序、蛋白印迹的检测及分析。结果壮医药线点灸降低了PHN大鼠背根神经节中肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6等炎症因子的mRNA和含量表达(均P<0.05)。RNA测序分析显示点灸组与模型组之间的差异基因在NF-κB信号通路显著富集,后续通过蛋白印迹的方法得到证实(均P<0.05)。与模型组相比,点灸组和PDTC组的p65、p-p65、NF-κB抑制蛋白(inhibitor of NF-κB,IκB)α和p-IκBα的蛋白表达水平显著降低(均P<0.05)。与点灸组相比,PDTC+点灸组的p65、p-p65、IκBα和p-IκBα的蛋白表达水平也显著降低(均P<0.05)。结论壮医药线点灸通过抑制NF-κB信号通路显著改善PHN大鼠的神经炎症。Objective To explore the therapeutic effect and mechanism of Zhuang medicine thread moxibustion on RTX induced postherpetic neuralgia(PHN)in rats.Methods 75 male SD rats were randomly divided into saline group,model group,moxibustion group,pyrrolidine dithiocarbamate(PDTC)group,and PDTC+moxibustion group.Except for the saline group,all other groups were intraperitoneally injected with resin toxin to establish the PHN like rat model.The moxibustion group was treated with Zhuang Medicine thread moxibustion every other day after successful modeling,once every other day,with 10 sessions as a course of treatment;After successful modeling of the PDTC group,intraperitoneal injection of NF-κB inhibitor PDTC 50 mg/kg once a day was administered;The PDTC+moxibustion group:After successful modeling,PDTC was injected into the abdominal cavity and treated with Zhuang medicine thread moxibustion therapy.Regularly measure the behavioral changes of rats in each group,and the method of RT-PCR,ELisa,RNA sequencing(RNA seq),Western blot(WB)were used to analysis the dorsal root ganglion tissue.Results Zhuang medicine thread moxibustion reduced the mRNA and content expression of inflammatory factors such as TNF-α,IL-1β,and IL-6 in the dorsal root ganglia of PHN rats(P<0.05).The result of RNA seq analysis showed that the difference between Zhuang medicine thread moxibustion and the model group were significantly enriched in the NF-κB signaling pathway,which was subsequently confirmed by Western blot(P<0.05).Compared with the model group,the protein expression levels of p65,p-p65,IκBα,and p-IκBαin the moxibustion group and the PDTC group were significantly decreased(P<0.05).Compared with the moxibustion group,the protein expression levels of p65,p-p65,IκBα,and p-IκBαin the PDTC+moxibustion group were significantly reduced(P<0.05).Conclusion Zhuang medicine thread moxibustion significantly improves neuroinflammation in PHN rats by inhibiting the NF-κB signaling pathway.

关 键 词：壮医药线点灸 带状疱疹后遗神经痛大鼠模型 核因子-ΚB信号通路 背根神经节 转录组测序 炎症因子 

分 类 号：R245.8[医药卫生—针灸推拿学]