丘脑室旁核谷氨酸能神经元促进丙泊酚麻醉苏醒  

Glutamatergic neurons in paraventricular nucleus of the thalamus promote wakefulness during propofol anesthesia

作　　者：覃昶 高敬岩 付豹 Qin Chang;Gao Jingyan;Fu Bao(Department of Critical Care Medicine,Affiliated Hospital of Zunyi Medical University,Zunyi 563003,Guizhou,China;Guizhou Key Laboratory of Anesthesia and Organ Protection,Zunyi Medical University,Zunyi 563003,Guizhou,China)

机构地区：[1]遵义医科大学附属医院重症医学科,贵州遵义563003 [2]遵义医科大学贵州省麻醉与器官保护重点实验室,贵州遵义563003

出　　处：《中华危重病急救医学》2025年第2期140-145,共6页Chinese Critical Care Medicine

基　　金：国家自然科学基金(82060209,81760206)。

摘　　要：目的通过行为学和脑电图(EEG)记录结合的方式,明确丘脑室旁核(PVT)谷氨酸能神经元是否参与丙泊酚诱导的意识状态改变。方法选择8~12周龄健康雄性VGluT2-IRES-Cre小鼠作为实验对象。①选择性毁损PVT中的谷氨酸能神经元,并观察其对丙泊酚麻醉诱导时间、麻醉苏醒时间及EEG不同频段能量的影响。②通过光遗传学选择性激活或抑制PVT中的谷氨酸能神经元,并观察其对丙泊酚麻醉诱导时间、麻醉苏醒时间及EEG不同频段能量的影响。结果①毁损PVT中的谷氨酸能神经元能够显著延迟小鼠丙泊酚麻醉苏醒时间,与对照组比较差异有统计学意义(s:409.43±117.49比273.71±51.52,P<0.05),但对麻醉诱导时间并无显著影响。在丙泊酚麻醉恢复阶段,毁损PVT中的谷氨酸能神经元的δ波(1~4 Hz)频段能量较对照组显著增加,β波(12~15 Hz)频段能量则显著减少。②通过光遗传学激活PVT中的谷氨酸能神经元能够显著延长丙泊酚麻醉诱导时间(s:161.67±29.09比119.33±18.98,P<0.05),同时显著缩短麻醉苏醒时间(s:208.67±57.19比288.83±34.52,P<0.05)。在丙泊酚麻醉诱导过程中,激活PVT中的谷氨酸能神经元能够使δ波和α波(8~12 Hz)频段能量显著降低,而在恢复阶段α波频段能量显著增加。③通过光遗传学抑制PVT中的谷氨酸能神经元能够显著延迟丙泊酚麻醉苏醒时间(s:403.50±129.06比252.83±45.31,P<0.05),但是对麻醉诱导时间并无显著影响。无论在丙泊酚麻醉诱导阶段还是恢复阶段,抑制PVT中的谷氨酸能神经元均能显著增加δ波频段能量。结论PVT谷氨酸能神经元参与调控丙泊酚麻醉苏醒过程。Objective To determine whether the glutamatergic neurons in the paraventricular nucleus of the thalamus(PVT)is involved in the change of consciousness induced by propofol through a combination of behavioral and electroencephalography(EEG)recordings.Methods Healthy male VGluT2-IRES-Cre mice aged 8-12 weeks were used in this experiment.①The glutamatergic neurons in the PVT was selectively damaged,and its effect on propofol anesthesia induction and recovery times as well as the energy of EEG in different frequency bands were observed.②Optogenetics was utilized to selectively activate or inhibit glutamatergic neurons in the PVT to assess their influence on anesthesia induction and recovery times under propofol as well as the energy of EEG in different frequency bands.Results①Selective ablation of glutamatergic neurons in the PVT significantly delayed recovery from propofol anesthesia with statistical difference as compared with the control group(s:409.43±117.49 vs.273.71±51.52,P<0.05),but had no significant effect on anesthesia induction time.During the recovery phase of propofol,selective ablation of glutamatergic neurons in the PVT exhibited higherδ-wave(1-4 Hz)power and reducedβ-wave(12-15 Hz)power as compared with the control group.②Optogenetic activation of glutamatergic neurons in the PVT significantly prolonged anesthesia induction time under propofol(s:161.67±29.09 vs.119.33±18.98,P<0.05)while significantly shortening the recovery time from propofol anesthesia(s:208.67±57.19 vs.288.83±34.52,P<0.05).During the induction phase of propofol,activation of glutamatergic neurons in PVT reduced δ-wave and α-wave(8-12 Hz)power,while during the recovery phase,α-wave power significantly increased as compared with the control group.③Optogenetic inhibition of glutamatergic neurons in the PVT delayed recovery from propofol anesthesia(s:403.50±129.06 vs.252.83±45.31,P<0.05),but had no significant effect on induction time.During both the induction phase and recovery phase of propofol,the optogenetic in

关 键 词：丘脑室旁核 丙泊酚 意识 谷氨酸 

分 类 号：R614[医药卫生—麻醉学]