樱桃果汁冻干粉通过调控尿酸转运蛋白减轻高尿酸性肾损伤  

Alleviation of Hyperuricemic Renal Injury Using Freeze-dried Cherry Juice Powder to Regulate Uric Acid Transporters

作　　者：余惠凡 周洁 黄林生 桂利利 吴正坤 李飞 YU Huifan;ZHOU Jie;HUANG Linsheng;GIO Lili;WU Zhengkun;LI Fei(School of Pharmaceutical Sciences,Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Hubei University of Medicine,Shiyan 442000,China;Department of Pharmacy,Gucheng County People's Hospital,Xiangyang 441000,China;Department of Hepatopancreatobiliary Surgery,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院药学院,武当特色中药研究湖北省重点实验室,湖北十堰442000 [2]谷城县人民医院药剂科,湖北襄阳441000 [3]十堰市太和医院肝胆胰外科,湖北十堰442000

出　　处：《现代食品科技》2025年第3期72-79,共8页Modern Food Science and Technology

基　　金：湖北省卫生健康委青年人才项目(WJ2021Q010);“十四五”湖北省高等学校优势特色学科群(生物与医药)项目(2022BMXKQT1)。

摘　　要：采用氧嗪酸钾连续腹腔注射7 d建立高尿酸血症(Hyperuricemia, HUA)小鼠模型,灌胃樱桃果汁冻干粉(Cherry Juice Freeze-Dried Powder, CJP)、苯溴马隆和别嘌呤醇干预HUA,试剂盒检测血清尿酸(Uric Acid,UA)、肌酐(Creatinine, Cr)、尿素氮(Blood Urea Nitrogen, BUN);苏木素-伊红染色观察肝脏、肾脏病理改变;Western Blot和免疫组化检测小鼠肾脏尿酸转运蛋白的表达水平。探究CJP通过调控尿酸转运蛋白减轻高尿酸性肾损伤作用。结果显示:CJP高剂量组小鼠UA水平由191.99μmol/L降低至113.12μmol/L(P<0.01)但仍高于正常水平25%,而Cr水平由16.09μmol/L降低至7.40μmol/L(P<0.01),BUN水平由8.24 mmol/L降低至4.73 mmol/L(P<0.01)均达到正常水平。肾脏组织损伤改善明显。CJP下调了尿酸重吸收蛋白尿酸盐转运体1(Urate Transporter1, URAT1)和葡萄糖转运蛋白9(Glucose Transporter 9, GLUT9),上调了尿酸分泌蛋白三磷酸腺苷结合盒转运体成员2(ATP-Binding Cassette Superfamily G Member 2, ABCG2)、阴离子转运蛋白-1(Anion Transporter 1, OAT1)、阴离子转运蛋白-3(Anion Transporter 1, OAT3)表达,对肝脏无明显毒性。因此,CJP可通过调控尿酸转运蛋白减轻高尿酸性肾损伤。该研究可为樱桃开发为防治尿酸性肾病的健康产品提供科学依据。A hyperuricemia(HUA)mouse model was developed through intraperitoneal injection of potassium oxonate for 7 days to investigate the potential of CJP in alleviating hyperuricemic renal injury by regulating uric acid transporters.After intervention in the HUA model using freeze-dried cherry juice powder(CJP),benzbromarone,and allopurinol,serum uric acid(UA),creatinine(Cr),and blood urea nitrogen(BUN)were detected using commercially available kits.Pathological changes in the liver and kidney were determined using hematoxylin-eosin(HE)staining.Expression of uric acid transporters in mouse kidney was assessed using western blot and immunohistochemistry.The UA level in the high-dose CJP group decreased from 191.99 to 113.12μmol/L(P<0.01),remaining 25%higher than the normal level,whereas the Cr level decreased from 16.09 to 7.40μmol/L(P<0.01),and the BUN level decreased from 8.24 to 4.73 mmol/L(P<0.01).The latter two indicators reached normal levels,demonstrating a substantial alleviation of renal tissue injury.CJP downregulated the expression of two uric acid-reabsorbing proteins,urate transporter 1 and glucose transporter 9,while upregulating the protein expression of three uric acid-secreting proteins,ATP-binding cassette superfamily G member 2,anion transporter 1,and anion transporter 3,with no obvious liver toxicity.CJP thus alleviates hyperuricemic renal injury by regulating uric acid transporters.This study can serve as a scientific basis for the development of health products derived from cherry for preventing and treating hyperuricemic renal injury.

关 键 词：樱桃果汁冻干粉 高尿酸血症 尿酸转运蛋白 高尿酸性肾损伤 肾保护 

分 类 号：R285.5[医药卫生—中药学] TS255.1[医药卫生—中医学]