Inhibition of UVB-induced Inflammatory Response and Regulation of Antimicrobial Peptide LL-37 by Polysaccharide Extracts from Saussurea laniceps  

雪莲多糖抑制UVB诱导的炎症反应及调控抗菌肽LL-37的作用研究

作　　者：MA Wenrui LIU Junchen ZHANG Hongyan LI Sunhua LI Li WANG Wei 马文睿;刘峻辰;张红艳;李孙华;李丽;王伟(北京植物资源研究开发重点实验室,北京100048;安赛搏(重庆)生物技术有限公司,重庆404100;润芳可(上海)生物科技有限公司,上海201400)

机构地区：[1]Beijing Key Laboratory of Plants Resource Research and Development,Beijing 100048,China [2]Acelbio(Chongqing)Biotechnology Co.Ltd.,Chongqing 404100,China [3]Revacl(Shanghai)Biotechnology Co.Ltd.,Shanghai 201400,China

出　　处：《现代食品科技》2025年第3期89-97,共9页Modern Food Science and Technology

摘　　要：In order to study the effects of Saussurea laniceps polysaccharides(SLPs)on the expression of inflammatory factors and antimicrobial peptide LL-37 in UVB-induced keratinocytes,SLPs were extracted by the ethanol thermal reflux method,and SLPs at different concentrations were used to examine the inhibitory effect of COX-2(a key mediator of inflammatory pathway).A cell model of UVB irradiation-induced inflammation was established to determine the influence of SLPs on prostaglandin E2(PGE-2),TNF-αand IL-1βinflammatory factors,as well as the relationships of SLPs with LL-37 expression.An enzyme-linked immunosorbent assay(ELISA)and western blot analysis were used to detect the production of inflammatory factors and LL-37 antimicrobial peptide.The results showed that the inhibition rate of COX-2 was 82.41%at 1000μg/mL,and the expression of PGE-2,TNF-αand IL-1βinflammatory factors in HaCaT cells was significantly downregulated at 100μg/mL(P<0.01).In addition,SLPs at 50μg/mL and 100μg/mL concentrations enhanced the expression of LL-37 antimicrobial peptide(P<0.01),thereby down-regulating the expression of TNF-αand IL-1βinflammatory factors,then reducing skin inflammation.Conclusion:SLP can significantly inhibit the inflammatory response induced by UVB,and can further slow down the damage caused by inflammation to the skin by regulating LL-37 antimicrobial peptides,which has the potential to prevent skin inflammatory damage caused by UVB irradiation.为研究雪莲多糖对UVB诱导角质形成细胞炎症因子表达及抗菌肽LL-37的影响,通过乙醇热回流方法提取雪莲多糖,使用不同浓度的雪莲多糖进行COX-2(炎症通路的关键介质)抑制效果检测。建立UVB照射诱导炎症细胞模型,测定其对前列腺素E2(PGE-2)、TNF-α和IL-1β炎症因子的影响以及雪莲多糖与LL-37表达的关系。采用酶联免疫吸附附实验(ELISA)和蛋白质印迹分析检测炎症因子和LL-37抗菌肽的产生。实验结果表明:雪莲多糖在1 000μg/m L质量浓度下COX-2抑制率达82.41%,100μg/mL质量浓度下显著下调HaCaT细胞中PGE-2、TNF-α和IL-1β炎症因子的表达(P<0.01)。此外50、100μg/mL质量浓度下雪莲多糖增强了LL-37抗菌肽的表达(P<0.01),从而下调TNF-α和IL-1β炎症因子表达以此减轻皮肤炎症。结论:雪莲多糖可以显著抑制由UVB诱导的炎症反应,并且可以通过调控LL-37抗菌肽进一步减缓炎症对皮肤带来的损伤,具有预防UVB照射引起皮肤炎症性损伤的潜力。

关 键 词：Saussurea laniceps ultraviolet B inflammatory factor antibacterial peptide LL-37 POLYSACCHARIDE 

分 类 号：R285.5[医药卫生—中药学] R285[医药卫生—中医学]