黄瓜新杆菌漆酶的表征及在工业染料脱色中的应用  

Characterization of Laccase from Neobacillus cucumis and Its Application in Industrial Dye Decolorization

作　　者：徐开凤 霍滢 郑穗平[1] XU Kaifeng;HUO Ying;ZHENG Suiping(School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006,China)

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《现代食品科技》2025年第3期107-114,共8页Modern Food Science and Technology

基　　金：国家重点研发计划项目(2021YFC2104001)。

摘　　要：漆酶具有染料脱色、织物表面改性和纺织品漂白的能力,因此在纺织工业中有着广泛的用途。筛选一种具有碱稳定性且适合纺织染料脱色应用的漆酶具有挑战性。该研究从黄瓜新杆菌(Neobacillus cucumis)中获得了一种新型碱性漆酶LacNC,并在大肠杆菌中进行了表达。LacNC是一种单体蛋白,其分子量大小为58 ku。LacNC以2,6-二甲氧基苯酚、丁香醛连氮(SGZ)和愈创木酚分别作为底物,其最适pH值分别为6.0、7.0和8.0。以愈创木酚为底物,LacNC最适温度为55℃。LacNC在pH值5.0~11.0范围内保持85%以上活性。在40℃孵育1 h后,保持80%以上活性。此外,LacNC对靛蓝胭脂红和孔雀石绿处理3 h,脱色率分别为90%和80%以上,对活性黑B的脱色率在24 h可达85%。综上所述,该研究获得了一种新型碱性漆酶,在碱性条件下对合成染料展现出较高的脱色效率,表明该漆酶是用于纺织工业废水处理的环保候选者。Laccase is widely used in the textile industry for dye decolorization,fabric surface modification,and textile bleaching.Screening for a laccase that is alkaline stable and suitable for textile dye decolorization is challenging.A novel alkaline laccase(LacNC)was obtained from Neobacillus cucumis and expressed in Escherichia coli.LacNC is a monomeric protein with a molecular weight of 58 ku.The optimum pH values for LacNC were 6.0,7.0,and 8.0 when 2,6-dimethoxyphenol,syringaldazine(SGZ),and guaiacol,respectively,were used as substrates.The optimal temperature for LacNC was 55℃ for the guaiacol substrate.The activity of LacNC remained more than 85%in the pH range of 5.0~11.0.After incubation at 40℃ for 1 h,the activity of LacNC was still higher than 80%.In addition,the decolorization rates of indigo carmine and malachite green by LacNC were more than 90% and 80% in 3 h,respectively,and the decolorization rate of reactive black B by LacNC reached 85%in 24 h.In this study,a new alkaline laccase demonstrating high decolorization efficiency for synthetic dyes under alkaline conditions was obtained.Hence,this laccase is an environmentally friendly candidate for treating wastewater in the textile industry.

关 键 词：基因挖掘 碱性漆酶 碱稳定性 纺织工业 染料脱色 

分 类 号：TS199[轻工技术与工程—纺织化学与染整工程] TS193.2[轻工技术与工程—纺织科学与工程]