加味过敏煎对抗生素加重过敏性哮喘小鼠气道菌群的影响  

The Effect of Modified Guomin Decoction(加味过敏煎)on the Airway Microbiota in Mice of Antibiotic Aggravated Allergic Asthma

作　　者：杨爽 王亚楠 黄燕 YANG Shuang;WANG Ya'nan;HUANG Yan(Inner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine,Hohhot Inner Mongolia 010000,China;Central Hospital of Yili Town,Oroqen Autonomous Banner,Hulun Buir Inner Mongolia 021000,China)

机构地区：[1]内蒙古自治区中医医院,内蒙古呼和浩特010000 [2]鄂伦春自治旗中蒙医院,内蒙古呼伦贝尔021000

出　　处：《中医药导报》2025年第3期18-23,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：内蒙古自治区人才开发基金高层次人才个人项目;2020年内蒙古自治区草原英才工程青年创新创业人才项目。

摘　　要：目的:探讨加味过敏煎对抗生素加重过敏性哮喘小鼠气道菌群的影响。方法:将40只雄性BALB/c小鼠随机分为空白组、抗生素加重过敏性哮喘组、加味过敏煎组(6.3 g/kg)、匹多莫德联合氨茶碱组(0.0378 g/kg、0.2 g/kg)。采用卵清蛋白(OVA)和氢氧化铝凝胶佐剂结合OVA雾化激发的方法建立过敏性哮喘小鼠模型,头孢哌酮舒巴坦钠滴鼻建立抗生素加重过敏性哮喘小鼠模型。雾化激发前30 min灌胃给药,连续给药7 d后采用16SrRNA测序检测气道菌群情况。结果:与空白组比较,抗生素加重过敏性哮喘组小鼠气道菌群丰富度Chaol及Observed-species指数、进化多样性Faith,s PD指数、均匀度Pielou_e指数及菌群多样性Shannon指数均降低(P<0.01);与抗生素加重过敏性哮喘组比较,加味过敏煎组小鼠气道菌群丰富度Chaol、Observed-species、Faith,s PD、Shannon均明显升高(P<0.01);空白组与抗生素加重过敏性哮喘组、匹多莫德联合氨茶碱组样品距离远,各组的菌群构成存在明显差异;空白组与加味过敏煎组的距离较近,其群落差异较小;抗生素加重过敏性哮喘组拟杆菌属、类拟杆菌属、普雷沃菌菌属、普劳瑟尔氏菌属相对比例升高;加味过敏煎组黄杆菌属、罗尔斯通菌属、链球菌属、红细菌属相对比例升高。结论:加味过敏煎可改善抗生素加重过敏性哮喘小鼠气道菌群失调情况,恢复气道菌群的多样性、丰富度及调节菌群结构。Objective:To investigate the effect of modified Guomin decoction on airway microflora in mice of antibiotic aggravated allergic asthma based on 16SrRNA sequencing technology.Methods:Totally 40 male BALB/c mice were randomly divided into blank group,antibiotic aggravated allergic asthma group(AAAA group),modified Guomin decoction group(6.3 g/kg),and pidotimod combined with aminophylline(0.0378 g/kg,0.2 g/kg)group(combined group).Allergic asthma mouse model was established by ovalbumin(OVA)and aluminum hydroxide gel adjuvant combined with OVA aerosol stimulation,and antibiotic aggravated allergic asthma model was established by cefoperazone sulbactam sodium nasal drip.The mice were administrated by gavage 30 minutes before nebulization,and airway microbiota was detected with 16SrRNA sequencing after 7 days of continuous administration.Results:Compared with the blank group,the airway flora richness Chaol,Observed species index,evolutionary diversity Faith,sPD index,evenness Pielou_e index and Shannon index decreased in AAAA group(P<0.01);Compared with AAAA group,Chaol,Observed-species,Faith,sPD and Shannon increased in modified Guomin decoction group(P<0.01).The samples of blank group were far away from those of AAAA group and combined group,and there were obvious differences in the composition of microbiota among each group.The distance between the blank group and modified Guomin decoction group was closer,and the community difference was small.At the generic level,the relative proportions of Bacteroides,Bacteroides,Prevotella and Prausselia in increased in AAAA group.The relative proportions of flavobacteria,Rollestonia,Streptococcus and rhodobacter increased in modified Guomin decoction group.Conclusion:Modified Guomin decoction could improve the imbalance of airway microbiota in mice of antibiotic aggravated allergic asthma,restore the diversity and richness of airway microbiota and regulate the structure of airway microbiota.

关 键 词：抗生素加重过敏性哮喘 气道菌群失调 加味过敏煎 小鼠 

分 类 号：R285.5[医药卫生—中药学]