蛋白质组学分析蕨麻胶囊对低温低氧下失血性休克大鼠的血清蛋白差异表达  

Differential Expression of Serum Proteins in Hemorrhagic Shock Model in Rats with Hypothermia and Hypoxia Treated with the Potentilla Anserina Capsule

作　　者：董伟 张改英[2] 刘瑞 王振华 曹忠平 代二庆 DONG Wei;ZHANG Gaiying;LIU Rui;WANG Zhenhua;CAO Zhongping;DAI Erqing(Tianjin University of Traditional Chinese Medicine,Tianjin 301617;Baotou Mongolian Medicine and Traditional Chinese Medicine Hospital,Baotou 014040;Chinese People's Armed Police Force Characteristic Medical Center,Tianjin 300162)

机构地区：[1]天津中医药大学,天津301617 [2]包头市蒙医中医医院检验、输血科,内蒙古包头014040 [3]中国人民武装警察部队特色医学中心,天津300162

出　　处：《临床输血与检验》2025年第2期207-216,共10页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：武警特色医学中心中医药服务能力培育与提升专项计划面上项目(No.2021ZY058);武警特色医学中心高原防治研究项目(No.TYGYFZZX04)资助。

摘　　要：目的采用数据非依赖采集(data-independent acquisition,DIA)蛋白质组学技术分析观察蕨麻胶囊对低温低氧下失血性休克大鼠血清蛋白差异表达的影响。方法22只雄性SD大鼠随机分为假手术组(n=8)、模型组(n=8)、治疗组(n=6)。3组大鼠适应性喂养1周,在造模前每日给予治疗组蕨麻胶囊稀释剂灌胃,假手术组和模型组大鼠灌胃同等剂量的生理盐水,连续灌胃7天。假手术组在另2组造模成功后同一时间段内搜集血清样本。所有血液样本通过蛋白质组学的方法,鉴定表达水平发生显著变化的蛋白质,并对其进行基因本体(gene ontology,GO)富集分析以确定其生物学功能,同时进行了京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路分析以探索它们所涉及的生物途径。结果共鉴定到3502个蛋白点。模型组与假手术组相比,差异表达明显的蛋白共196个,包含上调蛋白110个,下调蛋白86个。治疗组与模型组相比,差异表达明显的蛋白共104个,包括上调蛋白68个,下调蛋白36个。模型组与治疗组之间,Slfn2、Tex52、Fmod、Fabp5、Exoc3蛋白表达差异显著。通过差异蛋白的GO分析表明,差异蛋白主要涉及细胞过程、生物调节、应激反应、含蛋白复合物、催化活性、分子功能活性调节等。KEGG通路分析涉及核糖体、血小板活化、肌醇磷酸盐代谢、磷脂酰肌醇信号系统等通路。结论研究证实蕨麻胶囊可明显改变低温低氧下失血性休克大鼠血清相关免疫及炎性相关蛋白的表达,并可能通过核糖体代谢、血小板活化等代谢途径调节。Objective DIA proteomics technique was used to analyze the effect of Potentilla anserina capsule on the differential expression of serum protein in rats with hemorrhagic shock under hypothermia and hypoxia.Methods Twentytwo male SD rats were randomly divided into sham operation group(n=8),model group(n=8),and treatment group(n=6).The rats in the 3 groups were fed adaptively for 1 week.Before modeling,the rats in the treatment group were given Potentilla anserina capsule daily by intragastric administration,and rats in sham operation group and model group were given the same amount of saline for 7 days.Serum samples in the sham operation group were collected during the same time after the successful modeling of the other two groups.All blood samples were subjected to proteomics to identify proteins with significant changes in expression levels,and Gene Ontology(GO)enrichment analysis was performed to determine their biological functions.The Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis was also conducted to explore the biological pathways involved.Results A total of 3502 protein spots were identified.Compared with sham operation,196 proteins were significantly differentially expressed in the model group,including 110 up-regulated proteins and 86 down-regulated proteins.Compared with the model group,104 proteins were significantly differentially expressed in the treatment group,including 68 up-regulated proteins and 36 down-regulated proteins.The protein expressions of Slfn2,Tex52,Fmod,Fabp5 and Exoc3 were significantly different between the model group and the treatment group.The GO analysis of differentially expressed proteins showed that differential proteins were mainly involve cellular processes,biological regulation,stress response,protein-containing complexes,catalytic activity,and regulation of molecular functional activity,etc.KEGG pathway analysis involves pathways such as ribosome,platelet activation,inositol phosphate metabolism,and phosphatidylinositol signaling system.Conclusion The st

关 键 词：失血性休克 蕨麻胶囊 蛋白质组学 

分 类 号：R285.5[医药卫生—中药学] Q51[医药卫生—中医学]