消瘰丸合逍遥丸抑制FAS/FASL通路治疗大鼠桥本甲状腺炎  

Investigation on the treatment of Hashimoto’s thyroiditis in rats by inhibition of FAS/FASL pathway by Xiaoluo Pills and Xiaoyao Pills

作　　者：胡厚琴[1] 翟伟林 谢明[1] HU Houqin;ZHAI Weilin;XIE Ming(Department of Traditional Chinese Medicine,Guangzhou Development District Hospital,Guangdong,Guangzhou 510730,China)

机构地区：[1]广州开发区医院中医科,广东广州510730

出　　处：《中国医药科学》2025年第6期12-17,共6页China Medicine And Pharmacy

基　　金：广东省中医药局科研项目(20232122)。

摘　　要：目的 探索消瘰丸合逍遥丸抑制脂肪酸合成酶(FAS)/脂肪酸合成酶配体(FASL)通路治疗大鼠桥本甲状腺炎的分子机制。方法 24只SD大鼠随机分为对照组、模型组和治疗组,每组各8只。模型组和治疗组大鼠通过灌胃高碘水并注射免疫乳化剂以建立桥本甲状腺炎模型,对照组则给予等量蒸馏水。治疗组大鼠连续8周接受中药治疗。实验期间,监测大鼠的毛色、精神状态、饮食和活动状况等。采用ELISA法检测血清甲状腺球蛋白抗体(TGAb)、甲状腺过氧化物酶抗体(TPOAb)、游离三碘甲状腺原氨酸(FT3)、游离甲状腺素4(FT4)、促甲状腺激素(TSH)、肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-10、IL-6、IL-12含量,Western blot检测各组大鼠甲状腺组织FAS、FASL表达变化。结果 模型组大鼠血清中TPOAb、TGAb、FT3、FT4水平高于对照组,而TSH水平低于对照组,差异有统计学意义(P <0.05)。中药组大鼠血清中TPOAb、TGAb、FT3、FT4水平低于模型组,而TSH水平高于模型组,差异有统计学意义(P <0.05)。模型组大鼠血清中IL-6、TNF-α水平高于对照组,IL-12、IL-10水平低于对照组,差异有统计学意义(P <0.05)。中药组大鼠血清中IL-6、TNF-α水平低于模型组,IL-12、IL-10水平高于模型组,差异有统计学意义(P <0.05)。模型组大鼠甲状腺组织FAS、FASL蛋白表达显著高于对照组,而中药组大鼠甲状腺组织FAS、FASL蛋白表达显著低于模型组,差异有统计学意义(P <0.05)。结论 消瘰丸合逍遥丸能够通过降低FAS/FASL通路蛋白表达,抑制FAS/FASL通路激活,显著降低桥本甲状腺炎大鼠自身抗体滴度,并改善甲状腺功能,降低炎症反应。Objective To investigate the molecular mechanism of Xiaoluo Pills and Xiaoyao Pills by inhibition of the fatty acid synthase(FAS)/fatty acid synthase ligand(FASL)pathway in the treatment of Hashimoto’s thyroiditis in rats.Methods A total of 24 SD rats were randomly divided into the control group(n=8),the model group(n=8)and the treatment group(n=8).Rats in the model group and the treatment group were established with Hashimoto’s thyroiditis model by gavage of high iodine water and injection of immune emulsifier,while the control group was given an equal amount of distilled water.Rats of the treatment group received traditional Chinese medicine treatment for 8 consecutive weeks.During the experiment,the coat color,mental state,diet and activity of rats were monitored.ELISA method was used to detect serum anti-thyroglobulin antibodies(TGAb),thyroid peroxidase antibody(TPOAb),free triiodothyronine 3(FT3),free thiiodothyronine 4(FT4),thyroid stimulating hormone(TSH),tumor necrosis factorα(TNF-α),interleukin(IL)-10,IL-6,and IL-12 levels.Western blot was used to detect the expression changes of FAS and FASL in thyroid tissue of rats in each group.Results The levels of TPOAb,TGAb,FT3,and FT4 in the serum of rats in the model group were higher than those in the control group,while the TSH level was lower than that in the control group,with statistically significant differences(P<0.05).The levels of TPOAb,TGAb,FT3,and FT4 in the serum of rats in the traditional Chinese medicine group were lower than those in the model group,while the TSH level was higher than that in the model group,with statistically significant differences(P<0.05).In addition,the levels of IL-6 and TNF-αin the serum of rats in the model group were higher than those in the control group,while the levels of IL-12 and IL-10 were lower than those in the control group,with statistically significant differences(P<0.05).The levels of IL-6 and TNF-αin the serum of rats in the traditional Chinese medicine group were lower than those in the model group,wh

关 键 词：消瘰丸 逍遥丸 桥本甲状腺炎 FAS/FASL通路 

分 类 号：R259[医药卫生—中西医结合] R581.4[医药卫生—中医内科学]