慢性肾功能衰竭肠道微生态特征及排毒保肾丸的干预机制  

Gut Microbiota Characteristics in Chronic Kidney Disease and the Intervention Mechanism of Paidu Baoshen Pills

作　　者：傅奕 马伟 李鑫[3] 李艳梅 黄信介 伍宏泽 刘永芳 付义 FU Yi;MA Wei;LI Xin;LI Yan-mei;HUANG Xin-jie;WU Hong-ze;LIUYong-fang;FU Yi(Traditional Chinese Medicine Hospital of Jiang an County,Jiang an 644200,China;Center for Preventive Treatment of Diseases,Yulin Hospital of Traditional Chinese Medicine,Yulin 719000,China;Hunan Key Laboratory of Traditional Chinese Medicine Diagnostics,Hunan University of Traditional Chinese Medicine,Changsha 410208,China;Jiujiang Hospital of Traditional Chinese Medicine,Jiujiang 332000,China)

机构地区：[1]江安县中医医院,四川江安644200 [2]榆林市中医医院治未病中心,陕西榆林719000 [3]湖南中医药大学中医诊断学湖南省重点实验室,湖南长沙410208 [4]九江市中医院,江西九江332000

出　　处：《云南中医中药杂志》2025年第4期90-98,共9页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：江西省科技厅一般项目(S2016SFYBG0294);江西省卫生健康委科技计划(20204305);江西省中医药科研计划(2019A395)。

摘　　要：目的明确排毒保肾丸治疗慢性肾脏病(chronic kidney disease,CKD)的作用机制。方法SPF级雄性SD大鼠随机分为假手术组、模型组、排毒保肾丸组及尿毒清颗粒组4组。采用5/6肾切除模型建立CKD。成模后,排毒保肾丸组给予排毒保肾丸(0.54g/kg),尿毒清组给予尿毒清(1.35g/kg)治疗,假手术组和模型组予等容积蒸馏水,每天1次,连续8周。给药结束后,取肾及结肠,采用HE、PAS和Masson染色行病理学检查;取粪便,采用16S rDNA测序并进行生信分析。结果排毒保肾丸治疗可显著缓解5/6肾切除模型大鼠的肾脏和结肠病理损伤(P<0.05),并可调节肠道菌群,显著提高益生菌Prevotellaceae(普氏菌科)、Akkermansia(黏液杆菌)、Phascolarctobacterium(芽孢杆菌属)的相对丰度,同时显著降低Clostridiaceae(梭菌科)、Hungateiclostridiaceae(红毛菊科)、Ruminococcus(瘤胃球菌属)的相对丰度(P<0.05),表明排毒保肾丸通过调节肠道菌群,抑制炎症,修复肠道及肾脏组织病理,延缓5/6肾切除大鼠肾功能进展。结论排毒保肾丸通过调节肠道菌群,抑制炎症,修复肠道及肾脏组织病理,具有延缓CKD进展的作用。Objective:To elucidate the mechanism of Paidu Baoshen Pills in the treatment of chronic kidney disease(CKD).Methods:Male SPF-grade SD rats were randomly divided into four groups:sham surgery group,model group,Paidu Baoshen Pills group,and Niaoduqing Granules group.A 5/6 nephrectomy model was used to establish CKD.After model establishment,the Paidu Baoshen Pills group received Paidu Baoshen Pills(0.54 g/kg),the Niaoduqing group received Niaoduqing Granules(1.35 g/kg)and the sham surgery and model groups received an equal volume of distilled water,administered once daily for eight weeks.After treatment,kidney and colon tissues were collected for histopathological analysis by using HE,PAS and Masson staining.Fecal samples were subjected to 16S rDNA sequencing and bioinformatics analysis.Results:Paidu Baoshen Pills significantly alleviated renal and colonic pathological damage in 5/6 nephrectomized rats(P<0.05)and modulated gut microbiota composition.The treatment significantly increased the relative abundance of beneficial bacteria,including Prevotellaceae,Akkermansia,and Phascolarctobacterium,while significantly decreasing the relative abundance of Clostridiaceae,Hungateiclostridiaceae,and Ruminococcus(P<0.05).These findings suggest that Paidu Baoshen Pills exert their therapeutic effects by modulating gut microbiota,reducing inflammation and repairing intestinal and renal tissue damage,thereby slowing CKD progression.Conclusion:Paidu Baoshen Pills effectively regulate gut microbiota,inhibit inflammation and repair tissue damage in the intestines and kidneys,demonstrating potential in delaying CKD progression.

关 键 词：排毒保肾丸 慢性肾脏病 肠道菌群 作用机制 

分 类 号：R692[医药卫生—泌尿科学]