基于网络药理学探讨2型糖尿病合并下肢蜂窝组织炎经验方对2型糖尿病合并下肢蜂窝组织炎治疗机制  

Network Pharmacology Exploration of the Mechanism of Traditional Chinese Medicine Formula for Diabetic Foot with Lower Limb Cellulitis in Type 2 Diabetes Mellitus

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作  者:郭宪伟 曾姚姚 翁依华 林仕伟 何裕杰 GUO Xianwei;ZENG Yaoyao;WENG Yihua;LIN Shiwei;HE Yujie(Department of Surgery,Fuzhou Traditional Chinese Medicine Hospital,Fuzhou,Fujian 350001,China;Department of Comprehensive AreaⅠ,Fuzhou First General Hospital Dental Branch,Fuzhou,Fujian 350001,China)

机构地区:[1]福州市中医院外科,福建福州350001 [2]福州市第一总医院口腔专科院区综合一区,福建福州350001

出  处:《中国医药指南》2025年第11期1-4,共4页Guide of China Medicine

基  金:福州市卫生健康中青年科学研究项目(2021-S-wq30)。

摘  要:目的基于网络药理学探讨2型糖尿病合并下肢蜂窝组织炎经验方对2型糖尿病合并下肢蜂窝组织炎的治疗机制,明确其作用靶点及相关信号通路,为糖尿病合并感染性疾病的中医药治疗提供理论依据。方法利用TCMSP数据库,以口服生物利用度(OB)≥40%和类药性(DL)≥0.2为筛选标准,获取2型糖尿病合并下肢蜂窝组织炎经验方中的关键成分。通过CTD数据库获取经验方的非重复靶点,同时借助Genecards、Malacards、Disgenet和OMIM数据库,以特定搜索词获取2型糖尿病合并蜂窝组织炎的疾病靶点。将疾病靶点与经验方靶点取交集确定关键靶点,再运用STRING数据库构建关键靶点的蛋白互作(PPI)网络并进行分析,最后进行富集分析。结果筛选出2型糖尿病合并下肢蜂窝组织炎经验方的74个不重复关键成分。获取4697个经验方非重复靶点和29个可信度较高的疾病靶点,二者交集得到21个关键靶点。PPI网络分析显示,STAT3和PIK3R1处于网络关键核心位置,提示磷脂酰肌醇通路和STAT3信号可能是治疗的关键机理。同时,IL-6信号、IL-1信号、TNF信号和胶原生成信号在致病或抗病中发挥重要作用。富集分析表明,炎性信号、成骨分化、细胞迁移、糖尿病综合征AGE—RAGE信号等构成关键的致病和抗病机理。结论基于网络药理学的研究揭示了2型糖尿病合并下肢蜂窝组织炎经验方治疗2型糖尿病合并下肢蜂窝组织炎的潜在机制,明确了关键靶点、信号通路及相关作用机制,为该疾病的中医药治疗提供了参考依据,有助于后续相关辅助药物的研发及中医中药在临床的推广应用。Objective To explore the mechanism of a traditional Chinese medicine formula for diabetic foot with lower limb cellulitis in type 2 diabetes mellitus using network pharmacology,and to identify its key targets and related signaling pathways,providing a theoretical basis for the treatment of diabetes with infectious diseases using traditional Chinese medicine.Methods The key components of the diabetic foot formula were obtained from the TCMSP database using oral bioavailability(OB)≥40%and drug-likeness(DL)≥0.2 as screening criteria.Non-redundant targets of the formula were obtained from the CTD database,while disease targets for type 2 diabetes mellitus with cellulitis were obtained from Genecards,Malacards,Disgenet,and OMIM databases using specific search terms.The intersection of disease targets and formula targets was determined to identify key targets.A protein-protein interaction(PPI)network of key targets was constructed and analyzed using the STRING database,followed by enrichment analysis.Results A total of 74 non-redundant key components were screened from the diabetic foot formula.4697 non-redundant targets of the formula and 29 highly reliable disease targets were obtained,and the intersection of the two yielded 21 key targets.PPI network analysis showed that STAT3 and PIK3R1 were at the core of the network,suggesting that the phosphatidylinositol pathway and STAT3 signaling may be key mechanisms of treatment.Meanwhile,IL-6 signaling,IL-1 signaling,TNF signaling,and collagen synthesis signaling play important roles in both pathogenesis and anti-pathogenesis.Enrichment analysis showed that inflammatory signaling,osteogenic differentiation,cell migration,and diabetic syndrome AGE-RAGE signaling constitute key pathogenic and anti-pathogenic mechanisms.Conclusions Network pharmacology research revealed the potential mechanism of a diabetic foot formula for treating type 2 diabetes mellitus with lower limb cellulitis,identifying key targets,signaling pathways,and related mechanisms of action.This provides

关 键 词:2型糖尿病 下肢蜂窝组织炎 2型糖尿病合并下肢蜂窝组织炎经验方 网络药理学 

分 类 号:R587.1[医药卫生—内分泌]

 

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