响应面法优化添加太子参的云芝液体发酵培养基及发酵产物分析  

Optimization of the herbal medicine Radix pseudostellariae-contained medium in Trametes versicolor submerged fermentation and analysis of the fermentation products

作　　者：赵生月 张余 姜春玲 刘盛荣[2] 叶祖云 ZHAO Sheng-yue;ZHANG Yu;JIANG Chun-ling;LIU Sheng-rong;Ye Zu-yun(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Research Center of Fujian Province Characteristic Medicinal Plants,College of Life Science,Ningde Normal University,Ningde,Fujian 352100,China;College of Food Science and Engineering,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建省特色药用植物工程技术研究中心,宁德师范学院生物科学与工程学院,福建宁德352100 [3]福建农林大学食品科学学院,福建福州350002

出　　处：《宁德师范学院学报(自然科学版)》2025年第1期64-72,共9页Journal of Ningde Normal University(Natural Science)

基　　金：福建省科技厅对外合作项目(2021I0046);福建省科技厅高校产学合作项目(2021N5005);宁德师范学院科研项目(2022ZX01)。

摘　　要：为有效利用太子参资源,拓宽其应用领域,以太子参根须作为重要原料,利用响应面法优化云芝液体发酵培养基,并进行发酵产物活性成分分析。结果显示:以生物量为评价指标,优化的培养基为太子参21g·L^(-1)、蛋白胨11 g·L^(-1)、蔗糖15.81 g·L^(-1)、KH_(2)PO_(4)1.2 g·L^(-1)。菌丝体生物量、胞内三帖和胞内多糖含量,优化培养基均最高,分别为5.69、15.24×10^(-3)和0.58 g·L^(-1),比对照分别提高87.17%、85.63%和81.25%,比PDB培养基分别提高378.15%、361.82%和544.44%;发酵液的胞外多糖含量,以优化培养基发酵的为最高,达0.25 g·L^(-1),比对照提高127.27%,比PDB提高257.14%。菌丝生长和活性产物合成的结果表明:太子参根须是云芝深层发酵的良好底物,可促进云芝菌丝生长和活性产物合成。In order to effectively utilize the resources of Radix pseudostellariae,broaden the application field the medium composition with the root of R.pseudostellariae for Trametes versicolor submerged fermentation was optimized using response surface methodology,and the bioactive composition of the fermentation products were analyzed.With biomass as the evaluation index,the composition of medium was optimized,then the optimized media composition was defined as R.pseudostellariae root powder 21 g·L^(-1),11 g·L^(-1) peptone,15.81 g·L^(-1) sucrose,and 1.2 g·L^(-1)KH_(2)PO_(4).Using the optimized medium,control medium (replacement of R.pseudostellariae with sucrose),PDB medium for fermentation by T.versicolor,the optimized medium achieved the highest biomass,intracellular triterpenoids and intracellular polysaccharides at 5.69,15.24×10^(-3) and 0.58 g·L^(-1),respectively,which were 87.17%,85.63% and 81.25% higher than the corresponding values of the control medium and 378.15%,361.82% and 544.44% higher than those of PDB medium.The concentration of exopolysaccharides for the optimal medium was the highest,reaching 0.25 g·L^(-1),127.27% higher than that of the control medium and 257.14% higher than that of PDB medium.The results of mycelia growth and synthesis of active products showed that R.pseudostellariae root was a good substrate for deep fermentation and could promote mycelia growth and synthesis of active products.

关 键 词：太子参 云芝 液体发酵 培养基优化 

分 类 号：S567.53[农业科学—中草药栽培]