亳菊产黄酮内生真菌的分离筛选  

Isolation and Screening of Flavonoids Producing Endophytic Fungi from Chrysanthemum morifolium Ramat‘boju’

作　　者：郭慧 丁欢洛 杨雨佳 马婉露 于正阳 王岩 Guo Hui;Ding Huanluo;Yang Yujia;Ma Wanlu;Yu Zhengyang;Wang Yan(Bozhou University,Bozhou 236800,China)

机构地区：[1]亳州学院,安徽亳州236800

出　　处：《广东化工》2025年第6期60-63,共4页Guangdong Chemical Industry

基　　金：安徽省高等学校科学研究重点项目(2022AH052418);安徽省大学生创新创业训练项目(S202412926033)。

摘　　要：为了提高亳菊产黄酮的效率,采用亳州市谯城区十八里镇亳菊种植基地新鲜亳菊植株,对其进行消毒后取根、茎、叶、花,分离出亳菊内生真菌9株。通过薄层色谱和显色反应筛选出4个产黄酮菌株,并对产黄酮菌株进行总黄酮含量测定。通过形态学和分子生物学鉴定,得出菌株PHD、PJB、GHH和PHA均为链格孢属。其中产总黄酮能力最高的菌株是PHD,产量为7.735 mg/L;菌株PHA、PJB和GHH的黄酮产量分别为3.090 mg/L、2.376 mg/L和0.768 mg/L。本研究为亳菊内生真菌资源的开发利用奠定了基础。In order to improve the efficiency of flavonoid production,fresh Boju plants were taken from Boju planting base in Shibali Town,Qiaocheng District,Bozhou City.After disinfection,root,stem,leaf and flower were taken to isolate 9 Boju endophytic fungi.Four flavonoid strains were screened by TLC and chromogenic reaction,and the total flavonoid content was determined.Through morphological and molecular biological identification,the strains PHD,PJB GHH and PHA were identified as Alternaria.The strain with the highest flavonoids production capacity was PHD,which was 7.735 mg/L.The yield of PHA,PJB and GHH were 3.090 mg/L,2.376 mg/L and 0.768 mg/L,respectively.This study laid a foundation for the development and utilization of endophytic fungi resources in Chrysanthemum morifolium Ramat‘boju’.

关 键 词：亳菊 内生真菌 薄层色谱 显色反应 总黄酮 

分 类 号：TQ[化学工程]