水稻纹枯病拮抗内生菌的分离鉴定及防控效果  

Isolation and Identification of Antagonistic Endophytic Bacteria and Its Effect on Rice Sheath Blight

作　　者：蒋世琦 罗欣 陈柳 谢栋臣 孙淼 陈井生 肖国生 玄元虎 JIANG Shiqi;LUO Xin;CHEN Liu;XIE Dongchen;SUN Miao;CHEN Jingsheng;XIAO Guosheng;XUAN Yuanhu(College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing 404020,China;State Key Laboratory of Elemento-Organic Chemistry,Nankai University,Tianjin 300071,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,重庆404020 [2]南开大学元素有机化学国家重点实验室,天津300071

出　　处：《黑龙江农业科学》2025年第4期8-13,共6页Heilongjiang Agricultural Sciences

基　　金：重庆市自然科学基金(CSTB2023NSCQ-LMX0036);国家自然科学基金(32072406)。

摘　　要：水稻纹枯病严重危害水稻生产,为拓展水稻纹枯病的生防菌资源,从抗性品种水稻叶鞘中分离筛选获得对水稻纹枯病菌有生防效果的内生细菌。采用平板对峙试验、菌核萌发试验、离体叶片试验和盆栽试验,研究筛选得到的内生细菌对纹枯病菌室内和室外的防治效果。结果表明,筛选获得的12株内生菌中7株内生细菌对水稻纹枯病菌有显著的生防效果,其中5株对菌丝生长抑制效果超过55%。黏质沙雷菌Y3对水稻纹枯病菌菌丝生长和菌核萌发均有抑制效果,其对水稻离体叶片抑制率为39.21%,盆栽抑制率为29.62%,且对水稻无害。黏质沙雷菌Y3可以作为生物防治剂应用于水稻纹枯病生物防控。Rice sheath blight seriously jeopardizes rice production.In order to expand the resources of biocontrol bacteria against rice sheath blight,antagonistic endophytic bacteria were isolated and screened from leaf sheaths of resistant rice varieties.The control effects of antagonistic endophytic bacteria on rice sheath blight by in vitro leaf test,sterile seedling test,pot experiment,and field experiment.The results showed that seven of the 12 endophytic bacterial strains obtained from screening had significant biocontrol effects on rice blast fungus,and five of them inhibited mycelial growth by more than 55%.Serratia marcescens Y3 inhibited both mycelial growth and mycorrhizal germination of rice blight.Its inhibition rate was 39.21%in isolated leaves and 29.62%in potted rice plants,and it was harmless to rice.Serratia marcescens Y3 can be applied as a biocontrol agent for the biological control of rice blight.

关 键 词：内生细菌 水稻纹枯病 黏质沙雷菌 生物防控 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治] S435.111.4[农业科学—植物保护]