湿地植物对农村污水耐药基因遗传元件的富集  

Enrichment of antibiotic resistance genetic elements in rural sewage by wetland plants

作　　者：文菲菲 黄魁[1,2,3] 夏慧 宋炳昱 赵梦 WEN Fei-fei;HUANG Kui;XIA Hui;SONG Bing-yu;ZHAO Meng(School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;Key Laboratory of Yellow River Water Environment in Gansu Province,Lanzhou 730070,China;Ministry of Education Engineering Research Center of Water Resource Comprehensive Utilization in Cold and Arid Regions,Lanzhou 730070,China)

机构地区：[1]兰州交通大学环境与市政工程学院,甘肃兰州730070 [2]甘肃省黄河重点实验室,甘肃兰州730070 [3]寒旱地区水资源综合利用教育部工程研究中心,甘肃兰州730070

出　　处：《中国环境科学》2025年第4期1985-1994,共10页China Environmental Science

基　　金：甘肃省科技厅科技计划项目(22JR5RA335,22JR9KA034);甘肃省教育厅高校科研创新平台重大培育项目(2024CXPT-14)。

摘　　要：利用土壤生态渗滤系统处理农村污水,分析实验前后湿地植物鸢尾营养器官中整合酶基因intI1和转座酶基因tnpA-04的变化,探讨湿地植物对农村污水中MGEs的富集作用.结果表明,在60d的运行时间内,土壤生态渗滤系统对农村污水中氨氮和化学需氧量的平均去除率分别为88.50%和75.17%.鸢尾的平均高度和鲜重分别增加了3.63%和43.45%,且鸢尾组织内MGEs的含量增多了1.67ng/g,其中胞内MGEs占68.26%.而且鸢尾中tnpA-04基因的含量比intI1基因多37.86%,tnpA-04基因更容易在鸢尾营养器官中转移.植物营养器官中MGEs的生物富集能力表现为:茎>根>叶.此外,土壤的性质变化会显著影响植物对MGEs的富集(P<0.05).研究结果显示,湿地植物可有效富集农村污水中的MGEs,降低耐药基因的传播风险.This study aimed to explore the accumulation of MGEs by wetland plants in the treatment of rural sewage using a soil ecological infiltration system.Thus,the changes in the integrase gene intI1and transposase gene tnpA-04 in the vegetative parts of the wetland plant Iris were investigated before and after treatment.The results showed that,over a 60d operational period,the soil ecological infiltration system achieved average removal rates of ammonia nitrogen and chemical oxygen demand from rural sewage of 88.50%and 75.17%,respectively.The average height and fresh weight of the Iris increased by 3.63%and 43.45%,respectively.The concentration of MGEs in the plant increased by 1.67ng/g,with intracellular accounting for 68.26%.Forthermore,the abundance of the tnpA-04 gene was found to be 37.86%higher than that of the intI1gene,which demonstrated a higher propensity for transfer within the vegetative parts of Iris.The bioconcentration ability for MGEs in the plant's vegetative parts followed the order:stem>root>leaf.Moreover,variations in soil properties significantly influenced the plant's ability to accumulate MGEs(P<0.05).This study suggests that wetland plants can effectively accumulate MGEs from rural sewage,thereby reducing the risk of antibiotic resistance gene dissemination.

关 键 词：湿地植物 土壤渗滤 污水 水平转移 遗传元件 

分 类 号：X703[环境科学与工程—环境工程] X173