N-甲基吡咯烷酮(NMP)高效降解菌筛选及应用研究  

Screening and application of highly-efficient degrading strain for N-methyl-2-pyrrolidone(NMP)

作　　者：李宁宁 吕海慧 于得水 李雨桐 王晔 康建明 薛宇昕 金艳[1] 韩昫身 于建国[2,3] LI Ning-ning;LÜHai-hui;YU De-shui;LI Yu-tong;WANG Ye;KANG Jian-ming;XUE Yu-xin;JIN Yan;HAN Xu-shen;YU Jian-guo(National Engineering Research Center for Integrated Utilization of Salt Lake Resources,East China University of Science and Technology,Shanghai 200237,China;Joint International Laboratory for Potassium and Lithium Strategic Resources,East China University of Science and Technology,Shanghai 200237,China;School of Chemical Engineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学国家盐湖资源综合利用工程技术研究中心,上海200237 [2]华东理工大学钾锂战略资源国际联合实验室,上海200237 [3]华东理工大学化工学院,上海200237

出　　处：《中国环境科学》2025年第4期2305-2313,共9页China Environmental Science

基　　金：国家自然科学基金青年科学基金资助项目(52300085);中央高校基本科研业务费专项基金资助项目(JKB01241707);国家级大学生创新创业训练计划项目(202310251078,202410251078)。

摘　　要：以N-甲基吡咯烷酮(NMP)为唯一碳源和氮源,从锂离子电池阴极浆料搅拌装置清洗废水中筛选获得一株NMP高效降解菌NCSL-HH10,经16S r DNA测序及系统发育树分析,该菌属于污染伯克霍尔德氏菌.实验表明,该菌可在1500mg/LNMP废水体系,48h内实现100%NMP去除及94.3%TOC去除,高矿化度说明该菌具有较为完整的NMP降解路径.此外,该菌可在高达15000mg/LNMP体系实现NMP完全降解(100%),达到国内外文献报道最高的NMP降解浓度,且可实现较高矿化度(63.2%).选取10000mg/L NMP废水作为进水,发现Burkholderia contaminans NCSL-HH10可在开放体系60h内去除95.7%NMP及76.5%TOC,显著高于活性污泥(84h内去除39.0%NMP和30.2%TOC).N-methyl-2-pyrrolidone(NMP)was selected as the sole carbon and nitrogen source,and a strain NCSL-HH10 that could efficiently degrade NMP was isolated from the cleaning wastewater of lithium-ion battery cathode slurry mixer.16S rDNA sequencing and phylogenetic affiliation analysis showed that this strain belonged to Burkholderia contaminans.The results showed that 100%NMP removal and 94.3%TOC removal could be obtained in 1500mg/L NMP wastewater within 48h using this strain.Such a high mineralization degree indicated that the strain possessed a relatively complete NMP degradation pathway.In addition,the strain could completely degrade NMP with a concentration as high as 15000mg/L,which displayed the highest NMP degradation concentration with a high mineralization degree(63.2%)compared to the publicly available literatures.Finally,10000mg/L NMP wastewater was treated by Burkholderia contaminans NCSL-HH10 and activated sludge under open environment,respectively.It was found that 95.7%NMP and 76.5%TOC were removed within 60h by NCSL-HH10,which was significantly higher than activated sludge(only 39.0%NMP and 30.2%TOC were removed within 84h).

关 键 词：N-甲基吡咯烷酮(NMP) 锂离子电池 废水 生物降解 活性污泥 生物强化 

分 类 号：X703[环境科学与工程—环境工程]