芝麻素通过p38 MAPK/Nrf2/HO-1信号通路缓解大鼠急性肺损伤的作用研究  

Sesamin Alleviates Acute Lung Injury in Rats Through p38 MAPK/Nrf2/HO-1 Signaling Pathway

作　　者：刘金华 赵田甜[1] 胡根[1] LIU Jin-hua;ZHAO Tian-tian;HU Gen(The First Affiliated Hospital of Nanchang University,Nanchang,Jiangxi 330006)

机构地区：[1]南昌大学第一附属医院,江西南昌330006

出　　处：《实用中西医结合临床》2025年第6期1-4,12,共5页Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine

基　　金：江西省中医药管理局科技计划项目(编号:2021B703)。

摘　　要：目的探讨芝麻素对脂多糖(LPS)诱导大鼠急性肺损伤的保护作用及其作用机制。方法采用随机数字表法将大鼠分为空白对照组(Control组)、模型组(LPS组)及芝麻素干预组(LPS+SSM组),每组6只。Control组不做任何处理,LPS+SSM组按320 mg/kg灌胃芝麻素,LPS组按10 mL/kg灌浓度为0.5%的羧甲基纤维素钠,1次/d,连续喂养1周(造模前干预处理)。LPS组和LPS+SSM组采用气管注射LPS(5 mg/kg)建立急性肺损伤模型,造模成功6 h后取样。观察三组肺组织病理变化、炎症细胞因子、p38 MAPK磷酸化水平、核转录因子红系2相关因子2(Nrf2)及血红素加氧酶-1(HO-1)蛋白表达。结果HE染色显示,与Control组相比,LPS组大鼠肺泡结构受到破坏,肺泡间隙明显增厚,肺泡壁、肺泡腔或间质可见炎症细胞大量浸润;LPS+SSM组肺间质仅有轻微渗出,部分肺泡壁增厚,无透明膜形成,病理学损伤较LPS组减轻。大鼠肺组织湿/干重比(W/D)显示,Control组、LPS+SSM组W/D值均低于LPS组(P<0.05)。Control组、LPS+SSM组血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平均低于LPS组,Control组血清IL-1β、TNF-α水平均低于LPS+SSM组(P<0.05)。Western blot显示,Control组、LPS+SSM组大鼠肺组织p38 MAPK磷酸化水平低于LPS组,而Control组低于LPS+SSM组(P<0.05);Control组、LPS组Nrf2、HO-1均低于LPS+SSM组,而Control组低于LPS组(P<0.05)。结论芝麻素对LPS诱导的大鼠急性肺损伤发挥了显著的保护作用,其潜在机制可能与p38 MAPK/Nrf2/HO-1信号通路的激活有关。Objective To investigate the protective roles and mechanisms of sesamin on lipopolysaccharide(LPS)-induced acute lung injury in rats.Methods Rats were randomized into a blank control group(Control group),a model group(LPS group),and a sesamin intervention group(LPS+SSM group),with 6 rats in each group.The Control group did not receive any treatment,while the LPS+SSM group was given of sesamin(320 mg/kg)by gavage,and the LPS group was given 10 mL/kg of 0.5%carboxymethyl cellulose sodium by gavage,once a day,for one week(pre modeling intervention treatment).The LPS group and LPS+SM group established acute lung injury models by tracheal injection of LPS(5 mg/kg),and samples were taken 6 hours after successful modeling.Observed the pathological changes,inflammatory cytokines,p38 MAPK phosphorylation levels,expression of nuclear transcription factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)protein in three groups of lung tissues.Results The HE staining showed that compared with the Control group,the LPS group rats had damaged alveolar structures,significantly thickened alveolar spaces,and abundant infiltration of inflammatory cells in the alveolar walls,alveolar cavities or interstitium.The LPS+SM group had only slight exudation in the pulmonary interstitium,thickened of some alveolar walls,no formation of transparent membranes,the pathological damage was less than those in the LPS group.The wet/dry weight ratio(W/D)of lung tissue of rats showed that the W/D values of the Control group and LPS+SSM group were lower than that of the LPS group(P<0.05).The levels of serum interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in the Control group and LPS+SSM group were lower than those in the LPS group.The levels of serum IL-1βand TNF-αin the Control group were lower than those in the LPS+SSM group(P<0.05).Western blot showed that the phosphorylation levels of p38 MAPK in lung tissue of rats in the Control group and LPS+SSM group were lower than that in the LPS group,while the Control group was lo

关 键 词：急性肺损伤 芝麻素 脂多糖 p38 MAPK Nrf2/HO-1 

分 类 号：R285.5[医药卫生—中药学]