黄芪多糖对溃疡性结肠炎大鼠结肠病理损伤及Th17/Treg免疫平衡的影响  

Effect of Huangqi polysaccharides on colonic pathological injury and Th17/Treg immune balance in ulcerative colitis rats

作　　者：陈丹[1] 张立娜 高鹏[1] 张会峰 张靖波 CHEN Dan;ZHANG Lina;GAO Peng;ZHANG Huifeng;ZHANG Jingbo(Department of Clinical Laboratory,the 980th Hospital of the PLA Joint Logistic Support Force,Shijiazhuang,Hebei 050082,China)

机构地区：[1]中国人民解放军联勤保障部队第980医院检验科,河北石家庄050082

出　　处：《安徽医药》2025年第5期893-898,I0001,共7页Anhui Medical and Pharmaceutical Journal

基　　金：河北省医学科学研究课题计划项目(20200242)。

摘　　要：目的探究黄芪多糖对溃疡性结肠炎大鼠结肠病理损伤及辅助性T细胞17/调节性T细胞(Th17/Treg)免疫平衡的影响。方法于2022年1月至2023年1月,将60只大鼠按照随机数字表法分为对照组(NC组)、模型组(UC组),黄芪多糖低、中、高剂量组(HQDT-L组、HQDT-M组、HQDT-H组)和阳性对照组(SASP组)。观察大鼠一般情况;评估DAI评分;ELISA检测血清中白细胞介素6(IL-6)、白细胞介素10(IL-10)含量;检测大鼠髓过氧化物酶(MPO)活性;苏木精-伊红(HE)染色检测结肠组织病理学变化;流式细胞仪检测大鼠脾脏组织Treg/Th17比值;蛋白质印迹法检测结肠组织中维甲酸相关孤儿受体(ROPR-γt)和叉头框蛋白P3(FoxP3)蛋白表达。结果干预前,NC组大鼠DAI评分为0分,其余各组大鼠DAI评分明显升高(P<0.001),且各组间差异无统计学意义(P>0.05);干预后UC组大鼠DAI评分(2.87±0.56)分、血清中IL-6(25.61±10.83)ng/L含量、MPO活性(2.36±0.28)、结肠组织病理学评分(2.83±0.21)分、Th17细胞百分比(65.32±5.46)%及RORγt蛋白(0.21±0.03)表达明显高于NC组,血清中IL-10(6.89±0.74)ng/L含量、Treg细胞百分比(6.94±1.05)%和Treg/Th17比值(0.12±0.03)、FoxP3蛋白(0.15±0.02)表达明显低于NC组(P<0.001);和UC组相比,HQDT-L组、HQDT-M组和HQDT-H组大鼠DAI评分(2.16±0.32)分、(1.54±0.26)分、(1.12±0.17)分,血清中IL-6含量(201.34±8.15)ng/L、(141.57±7.42)ng/L、(75.32±6.85)ng/L,MPO活性(2.01±0.21、1.46±0.15、0.65±0.07),结肠组织病理学评分(2.24±0.19)分、(1.76±0.15)分、(1.08±0.12)分,Th17细胞百分比(52.11±4.32)%、(45.81±3.16)%、(31.26±2.54)%及RORγt蛋白(0.17±0.02、0.14±0.02、0.10±0.01)表达明显降低,血清中IL-10含量(8.36±0.81)ng/L、(10.27±0.94)ng/L、(13.81±1.05)ng/L,Treg细胞百分比(10.32±1.42)%、(17.61±1.53)%、(22.45±1.76)%和Treg/Th17比值(0.21±0.04、0.38±0.06、0.72±0.08)、FoxP3蛋白(0.31±0.04、0.55±0.06、0.69±0.07)表达明显升高(P<0.001);HQDT-H组和SASP组�Objective To investigate the effect of Astragalus polysaccharides on colonic pathological damage and immune balance of helper T cells 17/regulatory T cells(Th17/Treg)in rats with ulcerative colitis.Methods From January 2024 to January 2025,60 rats were randomly divided into a control group(NC group),a model group(UC group),low,medium,and high dose groups of Astragalus polysaccharides(HQDT-L group,HQDT-M group,HQDT-H group),and a positive control group(SASP group)according to a random number table method.the general condition of rats was observed;DAI score was evaluated;ELISA was used to detect the levels of interleukin-6(IL-6)and interleukin-10(IL-10)in serum;the activity of rat myeloperoxidase(MPO)was detected;Hematoxylin eosin(HE)staining was used to detect pathological changes in colon tissue;Flow cytometry was used to detect the Treg/Th17 ratio in rat spleen tissue;Western blot was used to detect retinoic acid associated orphan receptor(ROPR-γt)and forkhead box protein P3(FoxP3)protein expression in colon tissue.Results Before intervention,the DAI score of rats in the NC group was 0,while the DAI scores of rats in the other groups increased significantly(P<0.001),and there was no statistically significant difference between the groups(P>0.05).After intervention,the DAI score(2.87±0.56)points,IL-6 content(25.61±10.83)ng/L,MPO activity(2.36±0.28),colon histopathological score(2.83±0.21)points,Th17 cell percentage(65.32±5.46)%,and RORγt protein expression(0.21±0.03)in the UC group were significantly higher than those in the NC group.The IL-10 content(6.89±0.74)ng/L,Treg cell percentage(6.94±1.05),Treg/Th17 ratio(0.12±0.03),and FoxP3 protein expression(0.15±0.02)in the serum were significantly lower than those in the NC group(P<0.001).Compared with the UC group,rats in the HQDT-L group,HQDT-M group,and HQDT-H group had DAI scores of(2.16±0.32)points,(1.54±0.26)points,(1.12±0.17)points,serum IL-6 levels of[(201.34±8.15)ng/L,(141.57±7.42)ng/L,and(75.32±6.85)ng/L],MPO activity(2.01±0.21,1.46±0.15

关 键 词：黄芪 溃疡性结肠炎 结肠病理损伤 辅助性T细胞17/调节性T细胞免疫平衡 维甲酸相关孤儿受体/叉头框蛋白P3 

分 类 号：R285.5[医药卫生—中药学]