超声辅助胶束提取与富集喜树种子中金丝桃苷工艺研究  

Ultrasonic-Assisted Micellar Extraction and Enrichment of Hyperoside from Camptotheca Acuminata Seeds

作　　者：屈保玉 周晶 朱志尚 张卓[1] 王晓念 高雪霞 张铭[1] 孙镔琪 袁肖寒[2] 顾成波[1] Qu Baoyu(Key Laboratory of Forest Plant Ecology of Ministry of Education,Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-Based Active Substances,Engineering Research Center of Forest Bio-Preparation of Ministry of Education,College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin Heilongjiang 150040)

机构地区：[1]东北林业大学、化学化工与资源利用学院、森林植物生态学教育部重点实验室、林业生物制剂教育部工程研究中心、黑龙江省林源活性物质生态利用重点实验室,哈尔滨150040 [2]东北农业大学、生命科学与生物技术研究中心,哈尔滨150030

出　　处：《黑龙江医药》2025年第2期251-255,共5页Heilongjiang Medicine journal

基　　金：“十四五”国家重点研发计划项目课题“特色林源萜类和生物碱等新型医药中间体产品绿色制备技术”(编号:2022YFD2200805)。

摘　　要：目的:建立一种基于非离子型表面活性剂Genapol X-080结合超声辅助从喜树种子中提取和富集金丝桃苷的绿色提取工艺。方法:以金丝桃苷的提取率为衡量指标,对提取过程中Genapol X-080的浓度、料液比、超声功率、超声时间等因素进行单因素优化实验,同时对金丝桃苷富集过程中NaCl浓度进行了条件优化。结果:采用10%的Genapol X-080水溶液结合超声提取金丝桃苷,最佳工艺条件为料液比1∶60、超声功率480W、超声时间40min,在此条件下的金丝桃苷的提取率为1002.12μg/g;金丝桃苷的富集的最佳条件为8%的NaCl,此时金丝桃苷的富集率为82.09%。结论:使用Genapol X-080水溶液,成功实现了喜树种子中金丝桃苷的提取和富集,金丝桃苷提取率和富集率分别为1002.12μg/g和82.09%。Objective:Develop a green extraction process for extracting and enriching hyperoside from Camptotheca acuminata seeds through the utilization of the non-ionic surfactant Genapol X-080 in combination with ultrasonic assistance.Methods:With the extraction rate of hyperoside as the evaluation index,single-factor optimization experiments were carried out on factors such as the concentration of Genapol X-080,the solid-to-liquid ratio,the ultrasonic power,and the ultrasonic time during the extraction process.At the same time,the condition optimization of the NaCl concentration during the hyperoside enrichment process was also conducted.Results:The hyperoside was extracted using a 10%aqueous solution of Genapol X-080 combined with ultrasound,and the optimal process conditions were a solid-to-liquid ratio of 1∶60,an ultrasonic power of 480 W,and an ultrasonic time of 40min.Under these conditions,the extraction rate of hyperoside was 1002.12μg/g.The optimal condition for hyperoside enrichment was 8%NaCl,and at this time,the enrichment rate of hyperoside was 82.09%.Conclusion:Hyperoside was effectively extracted and concentrated from the seeds of Camptotheca acuminata with the application of Genapol X-080 aqueous solution.The achieved extraction rate and enrichment rate of hyperoside were precisely 1002.12μg/g and 82.09%,respectively.

关 键 词：Genapol X-080 超声波提取 富集 金丝桃苷 

分 类 号：R284.2[医药卫生—中药学]