仿刺参非受体酪氨酸激酶家族7个成员的鉴定及其表达模式  

Identification and expression pattern analysis of seven members of the non-receptor tyrosine kinase family in Apostichopus japonicus

作　　者：王纪萍 万俊杰 彭宇慧 赵雪琳 WANG Jiping;WAN Junjie;PENG Yuhui;ZHAO Xuelin(School of Marine Sciences,Ningbo University,Ningbo 315800,China;Key Laboratory of Aquacultural Biotechnology,Ministry of Education,Ningbo University,Ningbo 315800,China)

机构地区：[1]宁波大学海洋学院,浙江宁波315800 [2]宁波大学,水产生物技术教育部重点实验室,浙江宁波315800

出　　处：《水产学报》2025年第4期49-61,共13页Journal of Fisheries of China

基　　金：浙江省省属高校基本科研业务费专项(SJLY2023006)。

摘　　要：【目的】阐明仿刺参非受体酪氨酸激酶(SFKs)基因家族在病原胁迫响应中的功能。【方法】首先采用生物信息学方法,对仿刺参SFK基因家族成员的基因结构及进化关系进行分析,并通过实时荧光定量PCR(qRT-PCR)方法检测分析病原胁迫下仿刺参SFK基因家族成员的时空表达模式。【结果】本研究在仿刺参中共鉴定出SFK基因家族的7个成员,它们分属为SrcA、SrcB两个亚家族,不均匀地分布在3条染色体上。SFK蛋白结构域及motif分析结果显示,除SrcB-3外的6个成员都具有SH3结构域、SH2结构域、酪氨酸激酶核心结构域。其中,组成酪氨酸激酶核心结构域的5个基序在所有成员中广泛存在,不同成员SH3结构域及SH2结构域有相同的基序组成,表明AjSFK家族在进化上较为保守。SFKs在不同组织的基因表达模式分析表明,SFKs在仿刺参各个组织中广泛表达且具有明显的组织特异性,在肠道及触手中的表达量较高。另外,灿烂弧菌感染6 h后,SrcB-1~3的表达量极显著升高,体外脂多糖(LPS)胁迫24 h后SrcA-1和SrcB-2的表达量显著升高。【结论】仿刺参SFKs在响应病原刺激过程中具有较高的免疫响应且发挥重要作用。本实验是首个对仿刺参SFK基因家族进行系统识别及功能分析的研究,为仿刺参抗病研究提供了重要的理论基础。The SFK gene family is a family of non-receptor tyrosine kinases that function in apoptosis,phagocytosis,inflammation,and other immune processes in innate immunity.This study first analyzed the gene structure and evolutionary relationship of SFK gene family members of Apostichopus japonicus by bioinformatics methods to elucidate the function of the nonreceptor tyrosine kinases(SFKs)gene family in response to pathogen stress.qRT-PCR analyzed the temporal and spatial expression patterns of SFK gene family members under pathogen stress.In the present study,seven members of the SFK gene family were identified in A.japonicus,which were divided into two subfamilies SrcA and SrcB,distributed unevenly on three chromosomes.The domain and motif analysis showed that six members had conserved SH3 domain,SH2 domain and tyrosine kinase core domain except SrcB-3.Among them,five motifs that make up the core domain of tyrosine kinase existed widely in all members,and different members of the SH3 domain and SH2 domain had the same motif composition,indicating that the AjSFK family was relatively conservative in evolution.The analysis of SFKs gene expression patterns in different tissues showed that SFKs were widely expressed in various tissues of A.japonicus,and had apparent tissue specificity with high expression in the intestines and tentacles.In addition,the expression levels of SrcB-1-3 significantly increased after 6 h of infection with Vibrio splendidus,and the expression levels of SrcA-1 and SrcB-2 significantly increased after 24 h of LPS stress in vitro.The study showed that SFKs of A.japonicus had a high immune response and played an important role in responding to pathogen stimulation.This study was the first systematic identification and functional analysis of the SFK gene family in A.japonicus,and provided an important theoretical basis for disease resistance.

关 键 词：仿刺参 SFK基因家族 非受体酪氨酸激酶 基因表达 

分 类 号：Q786[生物学—分子生物学] S947[农业科学—水产养殖]