食物过敏伴便秘儿童肠道菌群变化与功能分析  

Changes and Functions Analysis of Intestinal Flora in Children with Food Allergy and Constipation

作　　者：吴慧 熊升华 黄从付 WU Hui;XIONG Shenghua;HUANG Congfu(Children’s Health Department,Panyu Maternal and Child Care Service Centre of Guangzhou,Guangzhou 511400,China;Department of Pediatrics,Longgang District Maternity&Child Healthcare Hospital of Shenzhen City//Longgang Maternity and Child Institute of Shantou University Medical College,Shenzhen 518100,China)

机构地区：[1]广州市番禺区妇幼保健院儿童保健科,广东广州511400 [2]深圳市龙岗区妇幼保健院儿科//汕头大学医学院龙岗妇幼临床学院,广东深圳518100

出　　处：《中山大学学报(医学科学版)》2025年第2期345-353,共9页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：深圳市龙岗区科技创新局基金(LGWJ2023-038);深圳市龙岗区妇幼保健院院级课题科研启动基金(Y2024011)。

摘　　要：【目的】运用高通量测序,了解食物过敏伴便秘儿童肠道菌群变化,并进行功能分析,为防治儿童食物过敏伴便秘提供依据。【方法】选取深圳市龙岗区妇幼保健院患有食物过敏伴便秘儿童33例作为疾病组(CPFA),选取59例该院儿童保健科的同龄健康儿童作为健康组(HC)。收集受试者的粪便标本,对标本进行16SrRNA基因高通量测序并分析。【结果】①HC组物种丰度高于CPFA组,两组肠道微生态物种群落多样性有差异;②门水平,与HC组相比,CPFA组拟杆菌门、脱硫杆菌门相对丰度低,厚壁菌门、放线杆菌门、变形杆菌门相对丰度高(P<0.05);③属水平,与HC组相比,CPFA组拟杆菌属、副拟杆菌属相对丰度低,双歧杆菌属、埃希氏-志贺氏菌属、韦荣氏球菌属相对丰度高(P<0.05);④功能分析显示,两组间存在显著差异的功能基因,与HC组相比,CPFA组在缬氨酸、亮氨酸和异亮氨酸的生物合成、半胱氨酸和蛋氨酸代谢、脂肪酸代谢、金黄色葡萄球菌感染等的功能基因丰度显著增加(P<0.05)。与胆汁酸生物合成、视黄醇代谢等有关的功能途径的功能基因丰度显著降低(P<0.05)。【结论】食物过敏伴便秘儿童肠道菌群及功能与健康儿童存在差异,这可能有助于探秘食物过敏伴便秘的发病机制,并为新的治疗干预提供理论基础。【Objective】To investigate the changes of intestinal flora in children with food allergy and constipation by high-throughput sequencing,and to analyze the function of intestinal flora,to provide a basis for the prevention and treatment of food allergy and constipation in children.【Methods】Totally 33 children with food allergy and constipation in Longgang District of Shenzhen were selected as the disease group(CPFA group),another 59 healthy children of the same age were recruited as the HC group.Stool samples were collected and subjected to high-throughput sequencing of 16SrRNA genes,followed by bioinformatics analysis.【Results】①The species abundance of HC group was higher than that of CPFA group,and the diversity of intestinal microbial community was different between the two groups;②At the phylum level,the relative abundance of Bacteroidota,Desulfobacterota in CPFA group was lower than that in HC group,and the relative abundance of Firmicutes,Actinobacteria and Proteobacteria in CPFA group was higher than that in HC Group(P<0.05);③At the genus level,the relative abundance of Bacteroids,Parabacteroides in the CPFA group was lower than that in the HC group,and the relative abundance of Bifidobacterium,Escherichia-Shigella and Veillonella in the CPFA group was higher than that in the HC group;④Functional analysis showed that there were significant differences in functional genes between the two groups.Compared with those in the HC group,the abundance of functional genes in valine,leucine and isoleucine biosynthesis,cysteine and methionine metabolism,fatty acid metabolism and Staphylococcus aureus infection were significantly increased in the CPFA group(P<0.05).The abundance of functional pathways related to bile acid biosynthesis and retinol metabolism was significantly decreased(P<0.05).【Conclusion】There are differences in intestinal flora and function between children with CPFA and HC,which may help to explore the pathogenesis of food allergy and constipation,and provide a theoretical basis

关 键 词：食物过敏 便秘 肠道菌群 菌群功能 高通量测序 

分 类 号：R725.7[医药卫生—儿科]