Embryotrophic effect of exogenous protein contained adipose-derived stem cell extracellular vesicles  

作　　者：Seonggyu Bang Ahmad Yar Qamar Sung Ho Yun Na‑Yeon Gu Heyyoung Kim Ayeong Han Heejae Kang Hye Sun Park Seung II Kim Islam MSaadeldin Sanghoon Lee Jongki Cho 

机构地区：[1]College of Veterinary Medicine and Research Institute for Veterinary Science,Seoul National University,Seoul 08826,Republic of Korea [2]College of Veterinary Medicine,Chungnam National University,Daejeon 34134,Republic of Korea [3]College of Veterinary and Animal Sciences,Jhang Sub-campus of University of Veterinary and Animal Sciences,Lahore 54000,Pakistan [4]Korea Basic Science Institute(KBSI),Ochang,Chungcheongbuk‑Do 28119,Republic of Korea [5]Viral Disease Research Division,Animal and Plant Quarantine Agency,Gimcheon,Gyeongsangbuk‑Do 39660,Republic of Korea [6]Department of Plastic and Reconstructive Surgery,Vascularized Composite Allotransplantation(VCA)Laboratory,Johns Hopkins School of Medicine,Baltimore,MD 21205,USA [7]Comparative Medicine Department,King Faisal Specialist Hospital&Research Centre,Riyadh 11211,Saudi Arabia

出　　处：《Journal of Animal Science and Biotechnology》2025年第2期596-609,共14页畜牧与生物技术杂志(英文版)

基　　金：The Ministry of Science and ICT through the National Research Foundation of Korea (NRF) supported this study (grant numbers:2021R1A2C2009294);The Research Institute for Veterinary Science at the Seoul National University partially funded this study。

摘　　要：Background Extracellular vesicles(EVs)regulate cell metabolism and various biological processes by delivering spe-cific proteins and nucleic acids to surrounding cells.We aimed to investigate the effects of the cargo contained in EVs derived from adipose-derived stem cells(ASCs)on the porcine embryonic development.Methods ASCs were isolated from porcine adipose tissue and characterized using ASC-specific markers via flow cytometry.EVs were subsequently extracted from the conditioned media of the established ASCs.These EVs were added to the in vitro culture environment of porcine embryos to observe qualitative improvements in embryonic development.Furthermore,the proteins within the EVs were analyzed to investigate the underlying mechanisms.Results We observed a higher blastocyst development rate and increased mitochondrial activity in early stage embryos in the ASC-EVs-supplemented group than in the controls(24.8%±0.8%vs.28.6%±1.1%,respectively).The terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assay of blastocysts also revealed signifi-cantly reduced apoptotic cells in the ASC-EVs-supplemented group.Furthermore,through proteomics,we detected the proteins in ASC-EVs and blastocysts from each treatment group.This analysis revealed a higher fraction of pro-teins in the ASC-EVs-supplemented group than in the controls(1,547 vs.1,495,respectively).Gene analysis confirmed that ASC-EVs showed a high expression of tyrosine-protein kinase(SRC),whereas ASC-EVs supplemented blastocysts showed a higher expression of Cyclin-dependent kinase 1(CDK1).SRC is postulated to activate protein kinase B(AKT),which inhibits the forkhead box O signaling pathway and activates CDK1.Subsequently,CDK1 activation influences the cell cycle,thereby affecting in vitro embryonic development.Conclusion ASC-EVs promote mitochondrial activity,which is crucial for the early development of blastocysts and vital in the downregulation of apoptosis.Additionally,ASC-EVs supply SRC to porcine blastocysts,thereby elon-gating the cell

关 键 词：Adipose-derived stem cell Embryonic development Extracellular vesicles PORCINE 

分 类 号：S823[农业科学—畜牧学]