Quercetin mitigates iron-induced cell death in chicken granulosa cell  

作　　者：Shuo Wei Felix Kwame Amevor Xiaxia Du Linxiang Li Zhixin Yi Gang Shu Yan Wang Xiaoling Zhao 

机构地区：[1]State Key Laboratory of Swine and Poultry Breeding Industry,College of Animal Science and Technology,Sichuan Agricultural University,Chengdu,Sichuan,P.R.China [2]Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,College of Animal Science and Technology,Sichuan Agricultural University,Chengdu,Sichuan 611130,China [3]Key Laboratory of Livestock and Poultry Multi‑omics,Ministry of Agriculture and Rural Affairs,Sichuan Agricultural University,Chengdu,Sichuan,P.R.China [4]Bazhong Academy of Agriculture and Forestry Sciences,Bazhong,P.R.China [5]Department of Basic Veterinary Medicine,Sichuan Agricultural University,Chengdu,Sichuan,P.R.China

出　　处：《Journal of Animal Science and Biotechnology》2025年第2期610-630,共21页畜牧与生物技术杂志(英文版)

基　　金：the Innovation and Demonstration of Industry and Education Integration in Feed Industrial Chain Transformation and Upgradation, Sichuan Province, China;the National Key R&D Program of China (Grant No. 2022YFD1600902-4);the National Natural Science Foundation of China (Grant No. 32272870 & 32350410427);the Sichuan Province Central Guided Local Science and Technology Development Special Programme (Grant No. 23ZYZYTS0346);the Sichuan Provincial Natural Science Foundation Outstanding Youth Science Fund Project (Grant No. 24NSFJQ0121);Breeding, Development, and Healthy Breeding of Plum Blossom Chickens in the Bazhong Mountains, a City School Cooperation Project in Bazhong City.

摘　　要：Background Granulosa cell(GC)apoptosis,ferroptosis,and other programmed cell death processes are markers of follicular aging.Quercetin has been shown to reduce ferroptosis,however,its effects on ferroptosis in poultry remains unexplored.Our preliminary study identified ferroptosis in aging ovaries.Therefore,in the present study,540-day-old Mountain Plum-blossom chickens were fed with quercetin supplementation at varying doses(0.2,0.4,and 0.6 g/kg),and examined its molecular effects on GC ferroptosis using an in vitro Erastin-induced model.Results The results showed that quercetin supplementation significantly increased egg production,which confirmed its potential to alleviate ferroptosis in chicken ovarian tissue.The in vitro experiment revealed that quercetin and Fer-1(positive control)mitigated Erastin-induced ferroptosis in GCs.Further,transcriptome analysis revealed that querce-tin modulated key genes such as acyl-CoA synthetase long-chain family member 4(ACSL4),solute carrier family 7 member 11(SLC7A11),and transferrin receptor(TFRC),involved in ferroptosis regulation.The results further showed that quercetin also reduced Erastin-induced apoptosis and inflammation by modulating the expression of genes and proteins related to apoptosis and inflammatory factors(NF-κB,TNF-α,IL-6,and IL-10).Conclusion Taken together,the results showed that quercetin improves egg production performance in chickens and mitigates ovarian ferroptosis in aging hens,and inhibits Erastin-induced ferroptosis,inflammation,and apoptosis in GCs.These findings revealed the protective role of quercetin in poultry ovarian tissue and its cellular mechanisms against detrimental factors in poultry production.

关 键 词：APOPTOSIS Cellular inflammation Ferroptosis Granulosa cell QUERCETIN 

分 类 号：S831[农业科学—畜牧学]