In vivo evaluation of selenium‑tellurium based nanoparticles as a novel treatment for bovine mastitis  

作　　者：Ludmila Kosaristanova Zuzana Bytesnikova Tatiana Fialova Jana Pekarkova Pavel Svec Frantisek Ondreas Vendula Jemelikova Andrea Ridoskova Peter Makovicky Ladislav Sivak Monika Dolejska Monika Zouharova Petr Slam Vojtech Adam Kristyna Smerkova 

机构地区：[1]Department of Chemistry and Biochemistry,Faculty of AgriSciences,Mendel University in Brno,Zemedelska 1665/1,Brno 61300,Czech Republic [2]Central European Institute of Technology,Brno University of Technology,Purkynova 656/123,Brno 61200,Czech Republic [3]Department of Microelectronics,Faculty of Electrical Engineering and Communication,Brno University of Technology,Technicka 3058/10,Brno 61600,Czech Republic [4]Contipro a.s.,Dolni Dobrouc 401,DolníDobrouc 56102,Czech Republic [5]Department of Histology and Embryology,Faculty of Medicine,University of Ostrava,Syllabova 9,Ostrava‑Vítkovice 70003,Czech Republic [6]Cancer Research Institute,Biomedical Research Center of the Slovak Academy of Sciences,Dúbravskacesta 9,Bratislava 84505,Slovak Republic [7]Central European Institute of Technology,University of Veterinary Sciences Brno,Palackeho tr.1946/1,Brno 61242,Czech Republic [8]Department of Biology and Wildlife Diseases,Faculty of Veterinary Hygiene and Ecology,University of Veterinary Sciences Brno,Palackeho tr.1946/1,Brno 61242,Czech Republic [9]Department of Microbiology,Faculty of Medicine,Charles University,Alej Svobody 76,Pilsen 32300,Czech Republic [10]Division of Clinical Microbiology and Immunology,Department of Laboratory Medicine,The University Hospital Brno,Jihlavska 20,Brno 62500,Czech Republic [11]Department of Infectious Diseases and Preventive Medicine,Veterinary Research Institute,Hudcova 296/70,Brno 62100,Czech Republic [12]Laboratory of Animal Immunology and Biotechnology,Department of Animal Morphology,Physiology and Genetics,Faculty of AgriSciences,Mendel University in Brno,Zemedelska 1665/1,Brno 61300,Czech Republic.

出　　处：《Journal of Animal Science and Biotechnology》2025年第2期668-684,共17页畜牧与生物技术杂志(英文版)

基　　金：Financial support from ERDF “Multidisciplinary research to increase application potential of nanomaterials in agricultural practice” (No.CZ.02.1.01/0.0/0.0/16_025/0007314);the assistance provided by the Research Infrastructure Nano Envi Cz, supported by the Ministry of Education, Youth and Sports of the Czech Republic under Project No. LM2018124;Czech Nano Lab Research Infrastructure supported by MEYS CR (LM2023051);Grant Agency of Gregor Johann Mendel (C-MNG-23–002);further supported by the Ministry of Agriculture of the Czech Republic by Grant RO0523;Internal Grant Agency of University of Veterinary Sciences Brno (223/2024/FVHE);the National Institute of Virology and Bacteriology project (Programme EXCELES, Project ID No. LX22NPO5103)-Funded by the European Union-Next Generation EU.

摘　　要：Background Bovine mastitis is one of the main causes of reduced production in dairy cows.The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus,whose resistant strains make the treatment of mastitis with conventional antibiotics very difficult and result in high losses.Therefore,it is impor-tant to develop novel therapeutic agents to overcome the resistance of mastitis-causing strains.In this study,novel selenium-tellurium based nanoparticles(SeTeNPs)were synthesized and characterized.Their antibacterial activity and biocompatibility were evaluated both in vitro and in vivo using a bovine model.A total of 10 heifers were divided into experimental and control groups(5 animals each).After intramammary infection with methicillin resistant S.aureus(MRSA)and the development of clinical signs of mastitis,a dose of SeTeNPs was administered to all quarters in the experimental group.Results Based on in vitro tests,the concentration of 149.70 mg/L and 263.95 mg/L of Se and Te,respectively,was used for application into the mammary gland.Three days after SeTeNPs administration,MRSA counts in the experimental group showed a significant reduction(P<0.01)compared to the control group.The inhibitory effect observed within the in vitro experiments was thus confirmed,resulting in the suppression of infection in ani-mals.Moreover,the superior biocompatibility of SeTeNPs in the organism was demonstrated,as the nanoparticles did not significantly alter the inflammatory response or histopathology at the site of application,i.e.,mammary gland,compared to the control group(P>0.05).Additionally,the metabolic profile of the blood plasma as well as the histology of the main organs remained unaffected,indicating that the nanoparticles had no adverse effects on the organism.Conclusions Our findings suggest that SeTeNPs can be used as a promising treatment for bovine mastitis in the pres-ence of resistant bacteria.However,the current study is limited by its small sample size,making it primarily a proof of t

关 键 词：ANTIBACTERIAL BIOCOMPATIBILITY HEIFER Intramammary Mammary gland MRSA NANOMATERIAL Resistance SeTe 

分 类 号：S858.23[农业科学—临床兽医学]