Proline metabolism is essential for alkaline adaptation of Nile tilapia(Oreochromis niloticus)  

作　　者：Minxu Wang Yuxi Yan Wei Liu Jinquan Fan Erchao Li Liqiao Chen Xiaodan Wang 

机构地区：[1]Laboratory of Aquaculture Nutrition and Environmental Health,School of Life Sciences,East China Normal University,Shanghai 200241,China

出　　处：《Journal of Animal Science and Biotechnology》2025年第2期714-728,共15页畜牧与生物技术杂志(英文版)

基　　金：supported by grants from the National Natural Science Foundation of China(No.32172946);the Young Elite Scientists Sponsorship Program by CAST(2022QNRC001);the Shanghai Rising-Star Program(2022);the Fundamental Research Funds for the Central Universities,ECNU。

摘　　要：Background Saline-alkaline water aquaculture has become a key way to mitigate the reduction of freshwater aquaculture space and meet the increasing global demand for aquatic products.To enhance the comprehensive utilization capability of saline-alkaline water,it is necessary to understand the regulatory mechanisms of aquatic animals coping with saline-alkaline water.In this study,our objective was to elucidate the function of proline metabolism in the alkaline adaptation of Nile tilapia(Oreochromis niloticus).Results Expose Nile tilapia to alkaline water of different alkalinity for 2 weeks to observe changes in its growth performance and proline metabolism.Meanwhile,to further clarify the role of proline metabolism,RNA interference experiments were conducted to disrupt the normal operation of proline metabolic axis by knocking down pycr(pyrroline-5-carboxylate reductases),the final rate-limiting enzyme in proline synthesis.The results showed that both the synthesis and degradation of proline were enhanced under carbonate alkalinity stress,and the environmental alkalinity impaired the growth performance of tilapia,and the higher the alkalinity,the greater the impairment.Moreover,environmental alkalinity caused oxidative stress in tilapia,enhanced ion transport,ammonia metabolism,and altered the intensity and form of energy metabolism in tilapia.When the expression level of the pycr gene decreased,the proline metabolism could not operate normally,and the ion transport,antioxidant defense system,and energy metabolism were severely damaged,ultimately leading to liver damage and a decreased survival rate of tilapia under alkalinity stress.Conclusions The results indicated that proline metabolism plays an important role in the alkaline adaptation of Nile tilapia and is a key regulatory process in various biochemical and physiological processes.

关 键 词：Alkalinity stress Oreochromis niloticus Oxidative stress Proline metabolism Pyrroline-5-carboxylate reductase 

分 类 号：S917.4[农业科学—水产科学]