Elevated levels of butyric acid in the jejunum of an animal model of broiler chickens: from early onset of Clostridium perfringens infection to clinical disease of necrotic enteritis  

作　　者：Hemlata Gautam Noor Ahmad Shaik Babajan Banaganapalli Shelly Popowich Iresha Subhasinghe Lisanework EAyalew Rupasri Mandal David S.Wishart Suresh Tikoo Susantha Gomis 

机构地区：[1]Department of Veterinary Pathology,Western College of Veterinary Medicine,University of Saskatchewan,52 Campus Drive,Saskatoon S7N 5B4,Canada [2]Department of Genetic Medicine,Faculty of Medicine,King Abdulaziz University,Jeddah 21589,Saudi Arabia [3]Department of Pathology and Microbiology,Atlantic Veterinary College,University of Prince Edward Island,550 University Ave,Charlottetown,PE C1A 4P3,Canada [4]Departments of Biological Sciences and Computing Science,University of Alberta,Edmonton,AB T6G 2E9,Canada [5]Vaccinology and Immunotherapy,School of Public Health,University of Saskatchewan,Saskatoon 7N 5E3,Canada

出　　处：《Journal of Animal Science and Biotechnology》2025年第2期758-776,共19页畜牧与生物技术杂志(英文版)

基　　金：support for the project was provided by Chicken Farmers of Saskatchewan(424357);Canadian Poultry Research Council(424854);Natural Sciences and Engineering Research Council of Canada(424679);Saskatchewan Agriculture Development Fund(426954).

摘　　要：Background Necrotic enteritis(NE)is an economically important disease of broiler chickens caused by Clostridium perfringens(CP).The pathogenesis,or disease process,of NE is still not clear.This study aimed to identify the alterations of metabolites and metabolic pathways associated with subclinical or clinical NE in CP infected birds and to investi-gate the possible variations in the metabolic profile of birds infected with different isolates of CP.Methodology Using a well-established NE model,the protein content of feed was changed abruptly before expos-ing birds to CP isolates with different toxin genes combinations(cpa,cpb2,netB,tpeL;cpa,cpb2,netB;or cpa,cpb2).Metabolomics analysis of jejunal contents was performed by a targeted,fully quantitative LC-MS/MS based assay.Results This study detected statistically significant differential expression of 34 metabolites including organic acids,amino acids,fatty acids,and biogenic amines,including elevation of butyric acid at onset of NE in broiler chickens.Subsequent analysis of broilers infected with CP isolates with different toxin gene combinations confirmed an eleva-tion of butyric acid consistently among 21 differentially expressed metabolites including organic acids,amino acids,and biogenic amines,underscoring its potential role during the development of NE.Furthermore,protein-metabolite network analysis revealed significant alterations in butyric acid and arginine-proline metabolisms.Conclusion This study indicates a significant metabolic difference between CP-infected and non-infected broiler chickens.Among all the metabolites,butyric acid increased significantly in CP-infected birds compared to non-infected healthy broilers.Logistic regression analysis revealed a positive association between butyric acid(coefficient:1.23,P<0.01)and CP infection,while showing a negative association with amino acid metabolism.These findings suggest that butyric acid could be a crucial metabolite linked to the occurrence of NE in broiler chickens and may serve as an early indica

关 键 词：Broiler chickens Butyric acid Gut health Metabolic pathways Necrotic enteritis Toxin genes 

分 类 号：S852.61[农业科学—基础兽医学]