Improvement of antioxidant capability by dietary N‑acetyl cysteine supplementation alleviates bone loss induced by chronic heat stress in finisher broilers  

作　　者：Huaiyong Zhang Herinda Pertiwi Joris Michiels Djoere Gaublomme Maryam Majdeddin Yuhuang Hou Matthieu Boone Dirk Elewaut Ivan Josipovic Jeroen Degroote 

机构地区：[1]Department of Animal Sciences and Aquatic Ecology,Laboratory for Animal Nutrition and Animal Product Quality,Ghent University,Ghent 9000,Belgium [2]College of Animal Science and Technology,Henan Agricultural University,Zhengzhou,Henan 450046,China [3]Unit Molecular Immunology and Inflammation,VIB Center for Inflammation Research,Ghent University and Department of Rheumatology,Ghent University Hospital,Ghent 9000,Belgium [4]Ghent University Centre for X-ray Tomography(UGCT),Ghent University,Ghent 9000,Belgium [5]Radiation Physics Research Group,Department of Physics and Astronomy,Ghent University,Ghent 9000,Belgium

出　　处：《Journal of Animal Science and Biotechnology》2025年第2期777-796,共20页畜牧与生物技术杂志(英文版)

基　　金：funded by the Ghent University Special Research Fund(BOF.PDO.2022.0002.01);Projects of International Cooperation of Henan Province(232102520016);National Natural Science Foundation of Henan Province(242300420159).

摘　　要：Background Heat stress(HS) incidence is associated with the accumulation of reactive substances, which might be associated with bone loss. N-Acetylcysteine(NAC) exhibits strong antioxidants due to its sulfhydryl group and being as the precursor for endogenous glutathione synthesis. Therefore, interplay between oxidative stress and bone turnover of broilers and the effects of dietary NAC inclusion on antioxidant capability and “gut-bone” axis were evaluated during chronic HS.Results Implementing cyclic chronic HS(34 ℃ for 7 h/d) evoked reactive oxygen species excessive production and oxidant stress, which was accompanied by compromised tibia mass. The RNA-seq of proximal tibia also revealed the enrichment of oxidation–reduction process and inflammatory outbursts during HS. Although no notable alterations in the growth performance and cecal microbiota were found, the diet contained 2 g/kg NAC enhanced the antioxidant capability of heat-stressed broiler chickens by upregulating the expression of Nrf2 in the ileum, tibia, and bone marrow. Simultaneously, NAC tended to hinder NF-κB pathway activation and decreased the m RNA levels of the proinflammatory cytokines in both the ileum and bone marrow. As a result, NAC suppressed osteoclastogenesis and osteoclast activity, thereby increasing osteocyte-related gene expression. Furthermore, the inclusion of NAC tended to increase the ash content and density of the whole tibia, as well as improve cortical thickness and bone volume of the diaphysis.Conclusions These findings HS-mediated outburst of oxidant stress accelerates bone resorption and negatively regulates the bone quality of tibia, which is inhibited by NAC in broilers.

关 键 词：Bone mass BROILERS Heat stress INTESTINE N-ACETYLCYSTEINE Oxidative stress 

分 类 号：S831.2[农业科学—畜牧学]