长鼻粗吻海龙中一个新天然产物化学成分研究  

Chemical Constituents of One New Natural Products of Trachyrhamphus longirostri

作　　者：陈浩 蔡月 徐春丽 刘贵金 邓世明[1] CHEN Hao;CAI Yue;XU Chun-li;LIU Gui-jin;DENG Shi-ming(Key Laboratory of Tropical Biological Resources of Ministry of Education,Hainan University/College of Pharmacy,Hainan University,Haikou 570228,China;Hainan Provincial Center for Disease Control and Prevention,Haikou 570203,China)

机构地区：[1]海南大学热带生物资源教育部重点实验室/海南大学药学院,海南海口570228 [2]海南省疾控中心,海南海口570203

出　　处：《中药材》2024年第7期1683-1687,共5页Journal of Chinese Medicinal Materials

基　　金：“十三五”海洋经济创新发展示范城市项目(HHCF20180103)。

摘　　要：目的:研究海龙科动物长鼻粗吻海龙Trachyrhamphus longirostri Kaup的化学成分。方法:采用硅胶柱层析、Sephadex LH-20凝胶柱和半制备HPLC对长鼻粗吻海龙85%乙醇提取物的石油醚萃取部位进行分离纯化,根据化合物理化性质和波谱数据进行结构鉴定。结果:从长鼻粗吻海龙中分离得到10个甾体类化合物,分别鉴定为:胆甾-3β,5α,6β-三醇-3-甲酸酯(1)、胆甾醇甲酸酯(2)、胆甾-4-烯-3-酮(3)、胆甾醇(4)、3β-羟基-胆甾-5-烯-7-酮(5)、6β-羟基-胆甾-4-烯-3-酮(6)、胆甾醇-5α,6α-环氧化物(7)、胆甾-5-烯-3β,7α-二醇(8)、胆甾-5-烯-3β,7β-二醇(9)、3β,5α,6β-三羟基胆甾烷醇(10)。结论:其中,化合物1为新天然产物,化合物2、5~7为首次从该属动物中分离得到。Objective:To study the chemical constituents of Trachyrhamphus longirostri.Methods:The petroleum ether extract of 85%ethanol extract of Trachyrhamphus longirostri was isolated and purified by silica gel column chromatography,Sephadex LH-20 gel column and semi-preparative HPLC.The structures of the compounds were identified according to the physiochemical properties and spectral data.Results:Ten steroid compounds were isolated from Trachyrhamphus longirostri and identified as cholestane-3β,5α,6β-tri-ol-3-formate(1),cholest-5-en-3-ol formate(2),cholesten-4-en-3-one(3),cholesterol(4),3β-hydroxy-cholest-5-en-7-one(5),6β-hy-droxy-cholest-4-en-3-one(6),cholesterol-5α,6α-epoxide(7),cholest-5-en-3β,7α-diol(8),cholest-5-en-3β,7β-diol(9),3β,5α,6β-tri-hydroxycholestane(10),respectively.Conclusion:Among them,compounds 1 is a new natural product,compounds 2 and 5 to 7 are iso-lated from Trachyrhamphus genus for the first time.

关 键 词：长鼻粗吻海龙 化学成分 结构鉴定 甾体 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]