关苍术化学成分研究  

Chemical Constituents of Atractylodes japonica

作　　者：王艺萌 孙延平[1] 祝明涛 白浩东 姬蓉 匡海学[1] WANG Yi-meng;SUN Yan-ping;ZHU Ming-tao;BAI Hao-dong;JI Rong;KUANG Hai-xue(Heilongjiang University of Chinese Medicine/Key Laboratory of Basic and Application research of Beiyao,Ministry of Education,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学/教育部北药基础与应用研究重点实验室,黑龙江哈尔滨150040

出　　处：《中药材》2024年第7期1700-1705,共6页Journal of Chinese Medicinal Materials

基　　金：国家中医药管理局中医药传承与创新“百千万”人才工程—岐黄工程首席科学家支持项目(国中医药人教函〔2021〕7号);国家中医药管理局中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者支持项目(国中医药人教函〔2018〕284号);2022年全国名老中医药专家传承工作室建设项目(国中医药人教函〔2022〕75号);第七批全国老中医药专家学术经验继承工作项目(国中医药人教函〔2022〕76号);黑龙江省“头雁”团队支持项目(黑龙江省头雁行动领导小组文件〔2019〕5号)。

摘　　要：目的:研究菊科苍术属植物关苍术Atractylodes japonica Koidz.ex Kitam.根茎的化学成分。方法:采用硅胶柱色谱、ODS柱色谱、制备型HPLC对关苍术根茎的75%乙醇提取物进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用脂多糖(LPS)诱导的小鼠巨噬细胞RAW 264.7体外炎症模型测定化合物对一氧化氮(NO)释放的抑制作用。结果:从关苍术中分离得到16个化合物,分别鉴定为:桉叶-4(15),7-二烯-3α,9β,11-三醇(1)、白术内酯Ⅶ(2)、过氧化白术内酯Ⅲ(3)、ent-(3R)-3-hydroxyatractylenolideⅢ(4)、4β,7β,11-enantioeudesmantriol(5)、双白术内酯(6)、脱落酸A(7)、1-acetoxytertadeca-6E,12E-dien-8,10-diyne-3-ol(8)、1,3-diacetoxytetradeca-6,12-dien-8,10-diyne(9)、(6E,12E)-tetradeca-6,12-dien-8,10-diyne-1,3-diol(10)、(6E,12E)-tetradecadiene-8,10-diyne-1,3-diol(11)、maricaffeolylide A(12)、绿原酸丁酯(13)、5-O-咖啡酰基奎宁酸丁酯(14)、(+)-jatrointelignan A(15)、threo-guaiacylglycerol-β-O-4′-dihydroconiferyl ether(16)。结论:其中,化合物12~16为首次从苍术属植物中分离得到,化合物1~5、7~9为首次从关苍术中分离得到。化合物1~3、8~11、13、14具有一定的抗炎活性。Objective:To study the chemical constituents of Atractylodes japonica rhizomes.Methods:The 75%ethanol extract of Atractylodes japonica rhizomes was isolated and purified by silica gel column chromatography,ODS column chromatography,preparative HPLC.The structures of the isolated compounds were identified according to physicochemical properties and spectral data.The inhibito-ry effect of the compounds on the release of nitric oxide(NO)was determined by using lipopolysaccharide(LPS)-induced inflammation model of mouse macrophage RAW 264.7 in vitro.Results:Sixteen compounds were isolated from Atractylodes japonica and identified as eudesm-4(15),7-diene-3α,9β,11-triol(1),atractylolideⅦ(2),peroxiatractylenolideⅢ(3),ent-(3R)-3-hydroxyatractylenolideⅢ(4),4β,7β,11-enantioeudesmantriol(5),biatractylolide(6),phellinene acid A(7),1-acetoxytertadeca-6E,12E-dien-8,10-diyne-3-ol(8),1,3-diacetoxytetradeca-6,12-dien-8,10-diyne(9),(6E,12E)-tetradeca-6,12-dien-8,10-diyne-1,3-diol(10),(6E,12E)-tetra-decadiene-8,10-diyne-1,3-diol(11),maricaffeolylide A(12),butyl chlorogenate(13),5-O-caffeoyl quinic acid butyl ester(14),(+)-jatrointelignan A(15),threo-guaiacylglycerol-β-O-4′-dihydroconiferyl ether(16),respectively.Conclusion:Among them,compounds 12 to 16 are isolated from the Atractylodes genus for the first time,compounds 1 to 5,7 to 9 are isolated from Atractylodes japonica for the first time,compounds 1 to 3,8 to 11,13,14 has certain anti-inflammatory activity.

关 键 词：关苍术 化学成分 分离鉴定 抗炎活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]