萌条香青的化学成分研究  

Chemical Constituents of Anaphalis surculosa

作　　者：吴兆梅 蔡昂 杨正江 郑定升 饶高雄 WU Zhao-mei;CAI Ang;YANG Zheng-jiang;ZHENG Ding-sheng;RAO Gao-xiong(Traditional Chinese Medicine College,Yunnan University of Chinese Medicine,Kunming 650500,China;Engineering Laboratory of Health Preservation Theory and Health Product with Ethnic Characteristics in Yunnan,Kunming 650500,China;Yunnan Jinbi Pharmaceutical Co.,Ltd.,Chuxiong 675400,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]云南省民族特色养生理论与健康产品工程研究中心,云南昆明650500 [3]云南金碧制药有限公司,云南楚雄675400

出　　处：《中药材》2024年第7期1712-1720,共9页Journal of Chinese Medicinal Materials

基　　金：云南省科技计划生物医药重大专项资助项目(2018ZF014-3-1)。

摘　　要：目的:研究菊科香青属植物萌条香青Anaphalis surculosa(Hand.-Mazz.)Hand.-Mazz.的化学成分。方法:综合利用硅胶、Sephadex LH-20葡聚糖凝胶、MCI gel、RP-C_(18)等色谱材料并结合重结晶等方法对萌条香青的50%乙醇提取物进行系统的分离纯化,根据单体化合物核磁共振1D和2D谱图,结合化合物的物理化学性质进行结构鉴定。结果:从萌条香青50%乙醇提取物中分离纯化得到44个化合物,分别鉴定为:5,7-二羟基-1(3H)-异苯丙呋喃酮(1)、platypterophathalide(2)、9-hydroxyeriobofuran(3)、5-methanol-7-hydroxy-2,2-dimethyl-2H-1-chromene-6-carboxylic acid(4)、helipyrone(5)、leontoaerialoside C(6)、4-[(6-O-(E)-咖啡酰基)-β-D-吡喃葡萄糖基]香草酸(7)、绿原酸(8)、异绿原酸A(9)、异绿原酸B(10)、异绿原酸C(11)、1,5-二咖啡酰奎宁酸(12)、桂皮酸(13)、E-香豆酸(14)、咖啡酸(15)、咖啡酸甲酯(16)、咖啡酸乙酯(17)、阿魏酸(18)、4-(3,4-二羟基苯基)-丁-2-酮(19)、苯甲酸(20)、对羟基苯甲酸(21)、香草酸(22)、藜芦酸(23)、7-methoxyisoylabrain(24)、6-[(5-甲基-6-乙基-4-羟基-吡喃酮-3-基)-亚甲基]光果伞素(25)、芹菜素(26)、木犀草素(27)、5,7-二羟基-3-甲氧基黄酮(28)、槲皮素(29)、槲皮素-3-O-β-D-吡喃葡萄糖苷(30)、芦丁(31)、熊果酸(32)、齐墩果酸(33)、坡曼酸(34)、β-谷甾醇(35)、胡萝卜苷(36)、邻苯二甲酸二正戊酯(37)、辛二酸二甲酯(38)、正二十一烷醇(39)、软脂酸-1-甘油酯(40)、二十四烷酸(41)、α-亚麻酸甲酯(42)、棕榈酸甲酯(43)、硬脂酸(44)。结论:所有化合物均为首次从萌条香青中分离得到。Objective:To study the chemical constituents of Anaphalis surculosa.Methods:The 50%ethanol extract of Anaphalis surculosa was systematically isolated and purified by MCI gel column,silica gel,RP-C_(18),Sephadex LH-20 and recrystallization methods.The structures were identified according to the 1D,2D NMR of the monomer compound and physicochemical properties.Results:44 compounds were isolated and purified from 50%ethanol extract of Anaphalis surculosa and identified as 5,7-dihydroxy-1(3H)-iso-benzofuranone(1),platypterophathalide(2),9-hydroxyeriobofuran(3),5-methanol-7-hydroxy-2,2-dimethyl-2H-1-chromene-6-carboxyl-ic acid(4),helipyron(5),leontoaerialoside C(6),4-[(6-O-(E)-caffeoyl)-β-D-glucopyranosyl]vanillic acid(7),chlorogenic acid(8),isochlorogenic acid A(9),isochlorogenic acid B(10),isochlorogenic acid C(11)1,5-dicaffeoylquinic acid(12),cinnamic acid(13),E-coumaric acid(14),3,4-dihydroxycinnamic acid(15),methyl caffeate acid(16),ethyl caffeate(17),ferulate(18),4-(3,4-dihydroxyphenyl)-butan-2-one(19),benzoic acid(20),p-hydroxybenzoic acid(21),vanillic acid(22),veratric acid(23),7-methoxy-isoylabrain(24),6-[(5-methyl-6-ethyl-4-hydroxy pyrone-3-yl)-methylene]glabranine(25),apigenine(26),luteoline(27),5,7-di-hydroxy-3-methoxyflavone(28),quercetine(29),quercetine-3-O-β-D-glucopyranoside(30),rutin(31),ursolic acid(32),oleanolic acid(33),polnolic acid(34),β-sitosterol(35),daucosterol(36),dipentyl phthalate(37),dimethyl suberate(38),1-heneicosanol(39),glycerol-1-hexadecanoate(40),lignoceric acid(41),α-linolenic acid methyl ester(42),methyl palmitate(43),stearic acid(44),re-spectively.Conclusion:All compounds are isolated from Anaphalis surculosa for the first time.

关 键 词：萌条香青 化学成分 分离鉴定 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]