力竭游泳联合大脑中动脉阻塞构建大鼠气虚血瘀型脑缺血再灌注模型的特征研究  

Study on the Characteristics of Qi Deficiency and Blood Stasis Type Cerebral Ischemia-reperfusion Model Induced by Exhaustive Swimming Combined with Middle Cerebral Artery Occlusion in Rats

作　　者：胡中吉 李艳玲[1] 邓常清[1] HU Zhongji;LI Yanling;DENG Changqing(Key Laboratory of Hunan Province of Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-cerebral Diseases,Hunan University of Traditional Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学中西医结合心脑疾病防治湖南省重点实验室,长沙410208

出　　处：《中国现代应用药学》2025年第4期521-528,共8页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金区域创新发展联合基金重点项目(U22A20377)。

摘　　要：目的基于中医“劳则气耗”理论,构建病证结合的气虚血瘀型脑缺血再灌注大鼠模型,分析其生物学特征。方法将大鼠随机分为假手术组、大脑中动脉阻塞(middle cerebral artery occlusion,MCAO)组和复合造模组。MCAO组采用线栓法阻塞左侧大脑中动脉2 h再灌注48 h;复合造模组先连续力竭游泳21 d,再同MCAO组进行缺血2 h再灌注48 h。Longa评分、气虚血瘀证量表评分评估模型的神经功能缺损和气虚血瘀证,2,3,5-氯化三苯基四氮唑(TTC)染色法、苏木素-伊红(HE)染色和尼氏(Nissl)染色观察脑组织病理形态变化,免疫荧光IgG法检测血脑屏障通透性,比色法检测脑组织三磷酸腺苷(adenosine triphosphate,ATP),Na^(+),K^(+)-ATP酶,二磷酸腺苷(adenosine diphosphate,ADP),丙二醛(malondialdehyde,MDA),总超氧化物歧化酶(total superoxide dismutase,T-SOD),ELISA法检测血清白细胞介素1β(interleukin-1β,IL-1β)、白细胞介素6(interleukin-6,IL-6)水平。结果与假手术组比较,MCAO组Longa评分和气虚血瘀证候评分显著增加(P<0.01),脑梗死率及脑水肿率增显著增加(P<0.01),海马CA1区病理损伤加重(P<0.01),尼氏体数量显著减少(P<0.01),血脑屏障IgG平均荧光强度显著增加(P<0.05或P<0.01);脑组织ATP、ADP含量,Na^(+),K^(+)-ATP酶活性显著降低(P<0.05或P<0.01);血清MDA、IL-1β、IL-6显著增加(P<0.05或P<0.01),T-SOD显著降低(P<0.01)。与MCAO组比较,复合造模组Longa评分和气虚血瘀证候评分显著增加(P<0.05或P<0.01),脑梗死率及脑水肿率显著增加(P<0.01)、血脑屏障IgG平均荧光强度显著增加(P<0.01);脑组织ATP、ADP含量,Na^(+),K^(+)-ATP酶活性显著降低(P<0.05或P<0.01);血清MDA、IL-1β、IL-6显著增加(P<0.05或P<0.01),T-SOD显著降低(P<0.01)。结论力竭游泳联合MCAO法可构建病证结合的气虚血瘀型脑缺血再灌注损伤大鼠模型,该法简单易行,兼具脑缺血和气虚血瘀的表征,相比单纯MCAO具有更重的神经功能缺损、气�OBJECTIVE To construct a rat model of Qi deficiency and blood stasis type cerebral ischemia/reperfusion based on the theory of"labor leads to Qi depletion"in traditional Chinese medicine,and analyze its biological characteristics.METHODS The rats were randomly divided into sham operation group,middle cerebral artery occlusion(MCAO)group,and composite modeling group.The MCAO group was blocked left middle cerebral artery by the wire embolus method for 2 h and reperfused for 48 h.The composite modeling group first underwent continuous exhaustion swimming for 21 d,followed by 2 h of ischemia and 48 h of reperfusion with the MCAO group.The neurological deficits of the model and the evidence of Qi deficiency and blood stasis were assessed by the Longa score and the Qi deficiency and blood stasis scale,and the pathomorphological changes of the brain tissue were observed by 2,3,5-triphenyltetrazolium chloride(TTC)staining,HE staining,and Nissl staining,and the permeability of the blood-brain barrier was detected by immunofluorescent IgG,and the brain tissue was examined for adenosine triphosphate(ATP),Na^(+),K^(+)-ATPase,adenosine diphosphate(ADP),malondialdehyde(MDA),total superoxide dismutase(T-SOD)by colorimetric assay,and serum interleukin-1β(IL-1β)and interleukin-6(IL-6)levels were detected by ELISA.RESULTS Compared with the sham operation group,the Longa score and Qi deficiency and blood stasis syndrome score were significantly increased in the MCAO group(P<0.01),the cerebral infarction rate and cerebral edema rate increased significantly(P<0.01),the pathological damage in the CA1 area of the hippocampus was aggravated(P<0.01),the number of Nissl bodies was significantly reduced(P<0.01),and the average fluorescent intensity of IgG at the blood-brain barrier was significantly increased(P<0.05 or P<0.01);the ATP and ADP content of brain tissue,Na^(+),K^(+)-ATPase activity were significantly decreased(P<0.05 or P<0.01);serum MDA,IL-1β,and IL-6 were significantly increased(P<0.05 or P<0.01),and T-SOD was significant

关 键 词：脑缺血 气虚血瘀型 模型 大鼠 能量代谢 氧化应激 炎症 

分 类 号：R965.2[医药卫生—药理学]