半夏泻心汤含药血清对胃癌细胞增殖和凋亡的影响及可能机制  

Effect and Mechanism of Banxia Xiexin Decoction Drug-Containing Serum on Proliferation and Apoptosis of Gastric Cancer Cells

作　　者：赵峥 石雪洋 李淑英 ZHAO Zheng;SHI Xue-yang;LI Shu-ying(School of Basic Medicine,North China University of science and Technology/Hebei Key Laboratory of Basic Medicine for Chronic Diseases,Tangshan 063000,China)

机构地区：[1]华北理工大学基础医学院/河北省慢性疾病基础医学重点实验室,河北唐山063000

出　　处：《中药材》2024年第9期2320-2327,共8页Journal of Chinese Medicinal Materials

基　　金：河北省自然科学基金资助项目(H2022209049)。

摘　　要：目的:探讨半夏泻心汤含药血清对人胃癌MGC-803细胞增殖、凋亡、NOS、PI3K、Akt mRNA及蛋白和p-PI3K、p-Akt蛋白表达的影响。方法:采用CCK-8法检测半夏泻心汤含药血清对MGC-803细胞增殖能力的影响,并筛选半夏泻心汤含药血清给药浓度。选择3个浓度的半夏泻心汤含药血清作为干预浓度考察其对人胃癌MGC-803细胞增殖、凋亡及PI3K/Akt信号通路的影响;将细胞分为空白对照组、半夏泻心汤含药血清组、空载对照组、NOS3过表达组、半夏泻心汤含药血清+NOS3过表达组,考察半夏泻心汤含药血清对NOS3过表达下人胃癌MGC-803细胞增殖、凋亡及PI3K/Akt信号通路的影响。Transwell和流式细胞术检测细胞侵袭和凋亡,ELISA检测细胞IFN-γ、IL-6、TNF-α水平,qPCR检测PI3K、Akt mRNA表达,Western Blot检测PI3K、p-PI3K、Akt、p-Akt蛋白表达。结果:与空白对照组比较,半夏泻心汤含药血清各浓度组细胞侵袭能力及IL-6、TNF-α水平显著降低,凋亡率及IFN-γ水平显著升高,PI3K、Akt mRNA及PI3K、p-PI3K、Akt、p-Akt蛋白表达显著降低(P<0.05)。与空载对照组比较,NOS3过表达组细胞侵袭能力及IL-6、TNF-α水平显著升高,凋亡率及IFN-γ水平显著降低,PI3K、Akt mRNA及PI3K、p-PI3K、Akt、p-Akt蛋白表达显著升高(P<0.05)。与NOS3过表达组比较,半夏泻心汤含药血清+NOS3过表达组细胞侵袭能力及IL-6、TNF-α水平显著降低,凋亡率及IFN-γ水平显著升高,PI3K、Akt mRNA及PI3K、p-PI3K、Akt、p-Akt蛋白表达显著降低(P<0.05)。结论:半夏泻心汤含药血清可通过抑制PI3K/Akt信号通路中NOS3的表达,抑制胃癌细胞增殖和侵袭,促进凋亡,发挥抗肿瘤的作用。Objective:To investigate the effects of Banxia xiexin decoction on proliferation,apoptosis,NOS,PI3K,Akt mRNA and protein,p-PI3K and p-Akt protein expressions in human gastric cancer MGC-803 cells.Methods:CCK-8 method was used to detect the the effect of Banxia xiexin decoction drug-containing serum on proliferation ability of MGC-803 cells,and the concentration of Banxia xiexin decoction drug-containing serum administration was screened.Three concentrations of Banxia xiexin decoction drug-containing se-rum were selected as intervention concentrations to investigate the effects on the proliferation,apoptosis and PI3K/Akt signaling pathway of human gastric cancer MGC-803 cells.The cells were divided into blank control group,Banxia xiexin decoction drug-containing serum group,no-loaded control group,NOS3 overexpression group,Banxia xiexin decoction drug-containing serum with NOS3 overexpression group to investigate the effects of Banxia xiexin decoction drug-containing serum on the proliferation,apoptosis and PI3K/Akt signaling pathway of human gastric cancer MGC-803 cells with NOS3 overexpression.The invasion ability and apoptosis were detected by Tran-swell and flow cytometry.The levels of IFN-γ,IL-6 and TNF-αin cells were detected by ELISA.The mRNA expressions of PI3K and Akt were detected by qPCR,and the protein expressions of PI3K,p-PI3K,Akt and p-Akt were detected by Western Blot.Results:Com-pared with blank control group,the cell invasion ability,IL-6 and TNF-αlevels were significantly decreased,the apoptosis rate and IFN-γlevel were significantly increased,and the PI3K and Akt mRNA and the PI3K,p-PI3K,Akt,p-Akt protein expressions were signifi-cantly decreased in Banxia xiexin decoction drug-containing serum concentration groups(P<0.05).Compared with no-load control group,the invasion ability,IL-6 and TNF-αlevels of cells in NOS3 overexpression group were significantly increased,the apoptosis rate and IFN-γlevel were significantly decreased,and the PI3K and Akt mRNA and the PI3K,p-PI3K,Akt,p-Akt protei

关 键 词：半夏泻心汤含药血清 胃癌 凋亡 磷脂酰肌醇3-激酶/蛋白激酶B 内皮型一氧化氮合酶3 

分 类 号：R285.5[医药卫生—中药学]