叶用芥菜BjBLH基因家族的鉴定及其在叶形变异中的表达分析  

Identification analysis of BjBLH gene family in Brassica juncea and analysis of its expression in leaf shape variation

作　　者：衡双平 朱心玉 何静娟 蔡佳钰 付梦珂 HENG Shuangping;ZHU Xinyu;HE Jingjuan;CAI Jiayu;FU Mengke(Collge of Life Sciences,Xinyang Normal University,Xinyang 464000,China)

机构地区：[1]信阳师范大学生命科学学院,河南信阳464000

出　　处：《信阳师范大学学报(自然科学版)》2025年第2期222-231,共10页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(32002056);河南省自然科学基金项目(232300420021)。

摘　　要：针对芥菜BLH基因家族成员是否参与调控芥菜叶形变异的生物学问题,采用生物信息学分析的方法,在全基因组水平鉴定和分析了芥菜BLH基因家族成员。通过基因表达分析,鉴定参与叶形变异的候选基因。研究结果发现,芥菜BLH基因家族主要包含45个家族成员,可分为13个亚家族。45个BLH基因家族成员在除了B07染色体上的其他17条染色体上不均匀分布,且均定位于细胞核。芥菜BLH基因启动子序列中包含多个与激素反应和非生物胁迫相关的顺式作用元件。芥菜BjBLH家族基因在不同组织中不同表达。其中,BjBLH4-1和BjBLH4-2在裂叶芥菜由内到外的第一、二、三片真叶中的表达含量显著高于锯齿叶芥菜叶片。这两个基因可能参与芥菜叶形的调控。Bioinformatic analysis was used to identify and analyze BLH gene family members of mustard leaf at the whole genome level,and aimed to answer the biological question of whether members of mustard BLH gene family participated in regulating leaf shape variation.After gene expression analysis,candidate genes involved in leaf shape variation were identified.The results showed that the identified 45 BLH genes can be divided into 13 subfamilies.The members of BLH gene family were unevenly distributed on 17 chromosomes except B07 chromosome and all of them were located in the nucleus.The analysis of cis-acting elements in the promoter region showed that the promoter sequence of BLH gene contained several cis-acting elements related to hormonal response and abiotic stress.Mustard BjBLH family genes were expressed differently in different tissues.Among them,the expression content of BjBLH4-1 and BjBLH4-2 in the first,second and third true leaves of split leaf mustard was significantly higher than that in sawtooth leaf mustard leaves.These two genes may be involved in the regulation of leaf shape of mustard.

关 键 词：芥菜 BLH 基因家族 生物信息学 基因表达 

分 类 号：Q37[生物学—遗传学]