福建省多黏菌素耐药基因mcr-1阳性鸡源大肠杆菌的流行性及耐药性分析  

Analysis on the prevalence and drug resistance of E.coli carrying polymyxin resistance gene mcr-1 isolated from chickens in Fujian Province

作　　者：戴婷婷 陈海玉 段楚楚 庄丽云 刘荣昌[3] 陈慧慧 余小珍 杨欣 陈梦诗 包银莉 程艳青 华宝玉[1,4] 黄翠琴 郑新添[1,4] DAI Ting-ting;CHEN Hai-yu;DUAN Chu-chu;ZHUANG Li-yun;LIU Rong-chang;CHENG Hui-hui;YU Xiao-zhen;YANG Xin;CHEN Meng-shi;BAO Yin-i;CHENG Yan-qing;HUA Bao-yu;HUANG Cui-qin;ZHENG Xin-tian(School of Life Science,Longyan University,Longyan 364012,China;College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Key Laboratory of Avian Disease Control,Institute of Animal Science and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China;Engineering Research Center of Zoonotic Diseases Prevention and Control,Fujian Universities,Longyan 364012,China)

机构地区：[1]龙岩学院生命科学学院,福建龙岩364012 [2]福建农林大学动物科学学院,福建福州350002 [3]福建省农业科学院畜牧兽医研究所,福建省禽病防治重点实验室,福建福州350003 [4]动物源性人兽共患病防控福建省高校工程研究中心,福建龙岩364012

出　　处：《中国预防兽医学报》2025年第1期9-16,共8页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金青年基金项目(32302883);动物源性人兽共患病防控福建省高校工程研究中心开放基金项目(2022K010);福建省中青年教师教育科研项目(JAT210434);上杭县奇迈科技创新基金项目(2019SHQM01);龙岩学院预防兽医学与生物技术福建省高等学校重点实验室开放基金项目(2022KF03)。

摘　　要：为了解福建省多黏菌素耐药基因mcr-1阳性大肠杆菌的流行情况和耐药性,本研究于2022年~2024年从福建省4个不同地区养殖场、屠宰场、菜市场的肉鸡和蛋鸡中分离到786株鸡源大肠杆菌,采用PCR鉴定mcr-1阳性大肠杆菌,统计不同地区、不同来源及不同种类鸡源mcr-1阳性大肠杆菌的检出率;采用微量肉汤稀释法测定mcr-1阳性大肠杆菌对7大类12种抗菌药物的敏感性;通过PCR检测mcr-1阳性菌的6大类12种耐药基因,利用卡方检验分析分离菌耐药表型和耐药基因之间的相关性;通过PCR鉴定分离菌中质粒的类型。结果显示,786株鸡源大肠杆菌中mcr-1基因的检出率为21.25%(167/786),以闽东地区(24.28%,42/173)、菜市场(25.41%,47/185)和蛋鸡(27.95%,64/229)中分离的mcr-1阳性大肠杆菌的检出率最高。药敏试验结果显示,分离菌对四环素类的多西环素(DOX)和四环素(TE)耐药的mcr-1基因阳性菌占比最高,分别为92.22%(154/167)和89.82%(150/167);对氨基糖苷类的庆大霉素(GEN)和氟喹诺酮类的环丙沙星(CIP)耐药的菌株最少,分别占44.91%(75/167)和56.29%(94/167);对氨基糖苷类、磺胺类、酰胺醇类、氟喹诺酮类、多黏菌素类、β-内酰胺类耐药的菌株占57.49%~73.65%。85.63%(143/167)的菌株为多重耐药菌(MDR)。不同地区、不同来源和不同种类鸡中分离的mcr-1阳性大肠杆菌的耐药性有差异;所测12种耐药基因均在mcr-1阳性菌中检出,其中四环素类的tet(A)(97.6%,163/167)、氨基糖苷类的aadA1(87.43%,146/167)、磺胺类的sul2(84.43%,141/167)、β-内酰胺类的blaTEM(81.44%,136/167)、氟喹诺酮类的qnrS(72.46%,121/167)及酰胺醇类的floK(67.66%,113/167)耐药基因的检出率均在50%以上;相关性分析显示,分离菌对氧氟沙星与其耐药基因qnrA、对环丙沙星与其耐药基因qnrS、对氨曲南和头孢他啶与其耐药基因blaTEM等均显著和极显著相关。分离菌主要携带IncI2型质粒(50.30%,84/167)、IncHI2型�To understand the prevalence and drug resistance of colistin-resistant gene mcr-1-positive E.coli in Fujian Province,in this study,786 strains of chicken-derived E.coli isolated from broilers and laying hens from farms,slaughterhouses and live poultry trading market in four different regions of Fujian Province from 2022 to 2024 were used.PCR was employed to identify mcr-1-positive E.coli,and the detection rates of mcr-1-positive E.coli from different regions,different sources and different types of chicken were counted.The sensitivity of mcr-1-positive E.coli to 12 kinds of antibiotic in 7 categories was determined by microbroth dilution method.Twelve drug resistance genes in 6 categories of mcr-1-positive bacteria were detected by PCR.The chi-square test was used to analyze the correlation between the drug resistance phenotypes and drug resistance genotypes of the isolated bacteria.The types of plasmids in the isolated bacteria were identified by PCR.The results showed that the detection rate of the mcr-1 gene in 786 strains of chicken E.coli was 21.25%(167/786).The highest detection rates of mcr-1 were found in the Eastern Fujian region(24.28%,42/173),live poultry trading markets(25.41%,47/185)and laying hens(27.95%,64/229).The results of drug susceptibility test indicated that among the mcr-1 gene-positive bacteria,the proportions of strains resistant to doxycycline(DOX)and tetracycline(TE)of the tetracycline class were the highest,which were 92.22%(154/167)and 89.82%(150/167),respectively.The strains resistant to gentamicin(GEN)of the aminoglycoside class and ciprofloxacin(CIP)of the fluoroquinolone class were the fewest,accounting for 44.91%(75/167)and 56.29%(94/167),respectively.The strains resistant to the aminoglycoside class,sulfonamide class,amphenicol class,fluoroquinolone class,polymyxin class andβ-lactam class accounted for 57.49%-73.65%.85.63%(143/167)of the strains were multi-drug resistant bacteria(MDR).There were differences in the drug resistance of mcr-1-positive E.coli isolated from different

关 键 词：大肠杆菌 多黏菌素 耐药基因 mcr-1 福建 鸡 

分 类 号：S852.61[农业科学—基础兽医学]